用PCR-SSCP检测母婴标本中沙眼衣原体的研究  

Detection of chlamydia trachomatis from maternal-neonatal specimens by PCR-SSCP.

作　　者：朱道银[1] 简丽[2] 夏云[1] 杨晓燕[1] 

机构地区：[1]重庆医科大学基础医学院微生物学教研室,630040 [2]重庆医科大学第一附属医院产科学教研室,630042

出　　处：《中国优生与遗传杂志》1998年第1期34-35,28,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金资助

摘　　要：用配对母胎标本探讨沙眼衣原体的垂直传播。材料与方法;用细胞培养、聚合酶链反应(PCR)和单链构象多态性(SSCP)检查配对母胎标本中的沙眼衣原体。结果:30份宫颈标本中8份沙眼衣原体培养和质粒 PCR 均为阳性:9名宫颈标本质粒 PCR 阳性的孕妇中有4份羊水标本培养和 PCR均为阳性:1例新生儿睑结膜和配对羊水标本表现相同的 SSCP 图谱。结论:孕妇的沙眼衣原体感染易导致胎儿感染;1例新生儿子宫内和出生后感染的沙眼衣原体为同一菌株。Investigation for vertical transmission of Chlamydia trachomatis by examining matched ma- ternal-fetal speaiments. Material and methods.Matched amniotic and cervial samples were examined to detect C.tra- chomatis by cell culture,PCR,and PCR-SSCP. Results.of 30 cervical samples 8 were positive for chlamydia by both culture and plasmid PCR.4 amniotie samples were positive for culture and PCR in 30 amniotic samples and in 9 matched cervical samples with positive PCR results.An amniotic sample and a conjunctival sample from the same newborne showed indentical profile of SSCP.Con- clusions.Pregnant women with chlamydial infec- tion readily led to fetal chlamydial infection.The same chlamydia strain from a newborne was con- firmed in uterus and after birth.

关 键 词：沙眼衣原体感染 生殖道感染 PCR-SSCP 妊娠 

分 类 号：R714.258[医药卫生—妇产科学] R374[医药卫生—临床医学]