以配合物为指示剂的新型DNA电化学传感器的研究  

Study on New DNA Electrochemical Biosensor with Metal Complex as the Hybridization Indicator

作　　者：逄介淳[1] 张纪梅[1] 代昭[1] 

机构地区：[1]天津工业大学材料科学与化学工程学院,天津300160

出　　处：《辽宁化工》2009年第1期1-4,共4页Liaoning Chemical Industry

摘　　要：利用自组装单分子膜法,将人工合成的GAPDH特征片断作为特异性探针固定在金电极表面,结合配合物指示剂Co(phen)23+构成DNA电化学传感器。采用红外吸收光谱对Co(phen)23+进行了表征,并利用差分脉冲伏安法研究了其电化学特性。结果表明,Co(phen)23+是一种特异性较强的指示剂,能够很好地嵌入到双链DNA的螺旋结构中,从而有效区分互补与非互补序列。The DNA electrochemical biosensor was prepared by self - assembling the thiol group modified GAPDH specific probe on the surface of Au electrode, metal complex as electro - active indicator. IR was employed to characterize the metal complex and differential pulse voltammogram （DPV） was used to investigate its electrochemical characteristics. The result shows that cobalt complex is a kind of indicator with high specificity.

关 键 词：DNA电化学传感器 探针 DNA杂交 自组装 电吸附 

分 类 号：R392[医药卫生—免疫学]