进境百合种球南芥菜花叶病毒的检测及分子鉴定  

Detection and molecular identification of Arabis mosaic virus from imported Lily hybridus

作　　者：刘毅[1,2] 丁元明 陈丽梅[1] 周剑 段禄华 和万忠 寸东义 刘忠善 

机构地区：[1]昆明理工大学生命科学与技术学院,650224 [2]云南省出入境检验检疫局

出　　处：《植物检疫》2009年第1期7-9,共3页Plant Quarantine

基　　金：国家科技支撑计划项目(2007BAD45B04)

摘　　要：本研究根据南芥菜花叶病毒(Arabismosaicvirus,ArMV)CP基因序列,设计并合成了特异性巢式PCR检测引物,通过第1轮RT-PCR和第2轮PCR扩增得到预期的930bp和260bp的条带,扩增序列与GenBank中登录的外壳蛋白基因存在86%～97%的同源性.结果表明百合上分离到的病毒为ArMV,进一步研究也证明巢式PCR灵敏度与普通RT-PCR相比高出了103倍。In this study, specific primers which were designed based on the sequence of CP gene in ArMV by nested PCR, and two amplified bands with expected sizes of 930bp and 260bp was obtained by the first RT - PCR and the second general PCR . The amplified ArMV sequence indicated that their identities with the ArMV CP gene registered in GenBank were 86% - 97%. All the results demonstrated that the isolated virus from Lily was ArMV, the sensitivity of nested PCR was higher than that of single RT - PCR. Further study showed that the detection limits of general RT - PCR was 103 times lower than that of nested PCR at least.

关 键 词：百合 南芥菜花叶病毒 RT—PCR 巢式PCR 

分 类 号：S41[农业科学—植物保护]