通经益髓方对实验性自身免疫性神经炎家兔协同刺激因子CD80/CD86调控作用的影响  

Regulating CD80/CD86 research on oral Tong-jing-yi-sui to EAN

作　　者：赵建军[1] 黎明全[1] 张炜煜[2] 任重[1] 王庆伟[1] 杨薇[1] 

机构地区：[1]长春中医药大学附属医院脑病科,130021 [2]长春中医药大学

出　　处：《环球中医药》2009年第1期27-30,共4页Global Traditional Chinese Medicine

基　　金：吉林省教育厅"十一五"科学技术研究课题:马钱子总生物碱治疗格林-巴利综合征量效关系及药效学研究编号:2007第74号

摘　　要：目的探讨通经益髓方对实验性自身免疫性神经炎家兔协同刺激分子CD80、CD86调控作用的影响。方法应用牛坐骨神经提取的髓鞘碱性蛋白Ⅱ(P2)蛋白免疫新西兰家兔,建立EAN(实验性自身免疫性神经炎)动物模型,采用不同方法进行治疗,抽血提取淋巴细胞,对各组家兔淋巴细胞协同刺激分子CD80、CD86蛋白表达水平进行统计学分析。结果治疗后,通经益髓方组和免疫球蛋白组CD80的蛋白表达水平明显降低(P<0.05,P<0.01),治疗7天后通经益髓方组降低程度不如免疫球蛋白组(P<0.05),至14天时通经益髓方组CD80的蛋白表达水平和免疫球蛋白组相比无明显差异(P>0.05),但均未达到正常水平(P<0.01);通经益髓方组和免疫球蛋白组CD86蛋白表达水平与模型组相比明显升高(P<0.01),治疗7天后通经益髓方组升高程度不如免疫球蛋白组(P<0.05),至14天时通经益髓方组CD86蛋白的水平和免疫球蛋白组相比无明显差异(P>0.05),但均未达到正常。结论通经益髓方组具有抑制CD80的分泌,促进CD86分泌的作用。使具有免疫抑制作用的Th2细胞分泌增加,发挥免疫下调作用,从而达到治疗EAN的作用。Objective To explore the effect of the prescription regarding Tong-jing-yi-sui on controlling function of allostimulatory factors from the rabbits with Experimental Autoimmune Neuritis. Methods New Zealand rabbits were immuned with myelin P2 lipid bindin abstracted from sciatic nerve of cattle so that Animal Models suffered in EAN（ Experimental Autoimmune Neuritis） were established. Lymphocyte were abstracted from the blood of experimental rabbits which were given different treatment and then the expressive levels of allostimulatory factors-CD80, CD86 protein in lymphocyte were statistically analyzed. Resuits The expressive levels of CD80 protein were obviously decreased （ P 〈 0. 05,P 〈 0. 01 ） in both group tong-jing-yi-sui and group immunoglobulin after treatment simultaneously they were higher in the former than the latter within 7days after treatment（ P 〈 0.05 ）, they have not variances between the two group at 14th day（ P 〉0.05 ） and were lower than normal levels（P 〈0.01 ）. The expressive levels of CD86 protein were more higher（ P 〈0.01 ） in both group tong-jing-yi-sui and group immunoglobulin than in control group after treatment simultaneously they were lower in the former than the latter within 7days after treatment （P 〈 0.05 ）, they have not variances between the two group at 14^th day （P 〉 0.05 ） and were higher than normal levels. Conclusions The prescription regarding Tong-jing-yi-sui has function in inhibitting CD80 secretion and promoting CD86 secretion, it can promote secretion of Th2 cell which has immunosuppressive action and it can play the role of immunity down regulation, accordingly it can accomplish treatment of EAN.

关 键 词：通经益髓方 实验性自身免疫性神经炎 协同刺激分子CD80/CD86 调控 

分 类 号：R285.6[医药卫生—中药学]