高效液相色谱法测定活血通络丸中紫丁香苷的含量  

Determination of Syringin in Huoxue Tongluo Pill by HPLC

作　　者：王刚 赵才德 马玉旬 

机构地区：[1]武威市药品检验所,甘肃武威733000 [2]武威市凉州区中医院,甘肃武威733000

出　　处：《中国中医药信息杂志》2009年第1期54-55,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立活血通络丸中紫丁香苷含量的测定方法。方法采用高效液相色谱法,色谱柱为KromasilC18(250mm×4.6mm,5μm),流动相为乙腈-1%冰醋酸(10∶90),流速为1.0mL/min,检测波长为270nm,柱温为室温,进样量为20μL。结果紫丁香苷浓度在5.3～53μg/mL范围内线性关系良好,平均回收率为98.29%,RSD=1.71%。结论方法简便、准确、重现性好,可作为活血通络丸的质量控制方法。Objective To establish a HPLC method for the content determination of syringin in Huoxue Tongluo Pill. Methods Chromatographic assay was performed on Kromasil C18 column （250mm×4.6mm,5μm） at room temperature, and the mixture solution of acetonitrile-1% aqueous acetic acid （10 ： 90, v/v） was used as mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was at 270 nm. The sample size was 20 μL. Results Good linear relationship was achieved when the concentration of syringin was in the range of 5.3-53 μg/mL. The average recovery was 98.29% with RSD= 1.71%. Conclusion The method is simple, accurate and the results are stable with good reproducibility. This method can be used for the content determination of syringin in Huoxue Tongluo Pill.

关 键 词：活血通络丸 高效液相色谱法 紫丁香苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]