南京椴茎段植株再生繁育技术  

Establishment of Regeneration System from the Stem of Tilia miqueliana

作　　者：刘芳 沈永宝[2] 游小英[3] 

机构地区：[1]北京土人景观与建筑规划设计研究院,北京100085 [2]南京林业大学森林资源与环境学院 [3]上海农林职业技术学院

出　　处：《林业科技开发》2009年第1期99-103,共5页China Forestry Science and Technology

基　　金：江苏省农业三项工程"南京椴繁殖技术开发与推广"(编号:sx(2006)120)

摘　　要：通过对南京椴无菌实生苗茎段的组织培养，探索南京椴带芽茎段分化、增殖及生根的最佳培养条件。结果表明，最适宜诱导南京椴不定芽分化的培养基为MS＋BA1．0mg／L＋NAA0．1mg／L＋蔗糖30．0g／L＋琼脂5．2g／L，诱导分化率为100％；最佳芽增殖及继代培养基为MS＋BA0．5—1．0mg／L＋NAA0．05mg／L＋蔗糖30．0g／L＋琼脂5．2g／L，繁殖系数达4．0～5．0；在生根培养基（1／2MS＋BA0．1mg／L＋IBA0．5mg／L＋蔗糖20．0g／L＋琼脂5．2g／L）中添加5．0g／LAC能明显促进不定根形成，40d后生根率达56．6％；生根苗移栽至泥炭土中，成活率达80．0％。A regeneration system of Tilia miqueliana was established by young stem segments of aseptic seedlings. The resuits showed that the most suitable medium for inducing adventitious buds was MS ＋ BA 1.0 mg/L ＋ NAA 0.1 mg/L ＋ sugar 30.0 g/L ＋ agar 5.2 g/L, with the differentiation rate of 100.0%. The most suitable medium for buds proliferation was MS ＋ BA 0.5 N 1.0 mg/L ＋ NAA 0.05 mg/L ＋ source 30.0 g/L ＋ agar 5.2 g/L , in which induction buds grew well and coefficient of multiplication reached 4.0 N 5.0 times. The rooting rate was 56.6% in the rooting medium （ 1/2MS ＋ BA 0.1 mg/L ＋ IBA 0.5 mg/L ＋ source 20.0 g/L ＋ agar 5.2 g/L ＋ AC 5.0 g/L） by 40 days cultivation. The survive rate of transplant was 80.0% in peat soil.

关 键 词：南京椴 组织培养 实生无菌苗 茎段 

分 类 号：S792.99[农业科学—林木遗传育种] S685.99[农业科学—林学]