文山山地乌骨鸡微卫星DNA多态性分析  

DNA Polymorphisms of Black-bone Chicken Population in Wenshan Mountanious Region Based on Microsatellite Markers

作　　者：苗永旺[1,2] 叶朗惠[1] 陈涛[1] 霍金龙[1] 李大林 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南大学,云南省生物资源保护与利用重点实验室,云南昆明650091 [3]云南省家畜改良工作站,云南昆明650021

出　　处：《云南农业大学学报（自然科学版）》2009年第1期63-66,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省教育厅科学研究基金项目(5Y0196B);国家自然科学基金项目(30660024);云南省应用基础研究重点项目(2007C0003Z);云南省应用基础研究计划面上项目(2006C0034M)

摘　　要：利用位于家鸡24条染色体上的33个微卫星标记对云南文山山地乌骨鸡50个个体进行了遗传多样性检测。共检测到118个等位基因，每个座位等位基因数目从2到9个不等，平均等位基因数为3．5758±1．5619，有效等位基因数在1．4274—6．1250之间，平均为2．9750±1．1389；群体平均表观杂合度、期望杂合度及平均多态信息含量分别为0．8927±0．2172，0．6214±0．1259和0．5447±0．1528。研究结果表明：文山山地乌骨鸡群体内存在丰富的遗传多样性。In the present paper, the microsatellite DNA polymorphisms of 50 individuals sampled from the chicken population of Wenshan mountanious region, Yunnan province, were assayed by using PCR and polyacrylaminde gel electrophoresis. Out of 33 microsatellite loci tested which are located on chicken＇s 24 chromosomes, a total of 118 alleles were detected, and the number of alleles varied from 2 to 9, while the number of effective alleles varied from 1. 427 4 to 6. 125 0, giving the mean value of 3.575 8 ± 1. 561 9 alleles and 2. 975 0 ±1. 138 93 effective alleles per locus. The average observed heterozygosity, expected heterozygosity and polymorphism information content were estimated as 0. 892 7± 0. 217 2, 0. 621 4 ±0. 125 9 and 0. 544 7 ±0. 152 8, respectively. The results revealed that the genetic variability in the chicken population analyzed was rich.

关 键 词：文山山地乌骨鸡 微卫星标记 遗传变异 多态信息含量 杂合度 

分 类 号：S831.2[农业科学—畜牧学]