益气活血方对球囊损伤大鼠颈总动脉后血栓形成的抑制作用研究  

Study on the inhibitory effect of yiqihuoxue prescriptions to experimental thrombus after carotid artery balloon injury in rats

作　　者：刘彬[1] 符逢春[1] 徐慧[1] 王萍[1] 林辉[1] 刘起胜[1] 

机构地区：[1]湖南中医药高等专科学校,株洲市412012

出　　处：《中国心血管病研究》2009年第1期49-51,共3页Chinese Journal of Cardiovascular Research

基　　金：湖南省教育厅科技处优秀青年项目（06B072）

摘　　要：目的观察损伤血管环氧化酶-1（COX-1）和诱导型一氧化氮合酶（iNOS）的表达情况，探讨益气活血方对球囊损伤大鼠颈总动脉后血栓形成的干预机制。方法随机将90只雄性SD大鼠分为模型组，阿司匹林组，益气活血方大、中、小剂量组。球囊损伤大鼠颈总动脉前给予高脂饲料喂养30d，造成高脂血症；然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉，造成颈总动脉管腔狭窄。手术前1d开始给药，每组动物于术后（5d、14d、28d）处死，取损伤颈总动脉，常规固定、包埋、切片，用免疫组织化学SABC法检测COX-1和iNOS的表达。结果血管损伤后模型组COX-1表达明显增多，术后应用中药益气活血方或阿司匹林后，COX-1表达明显减少，术后5d、14d和28d，用药各组（除中药小剂量组外）COX-1表达与模型组比较差异有统计学意义（P〈0．05）；术后用药各组iNOS表达明显增多，与模型组比较差异有统计学意义（P〈0．05）。结论益气活血方下调细胞COX-1的表达、上调iNOS表达，可能通过抑制TXA2产生和舒张血管作用而抑制血管内血栓形成防治再狭窄。Objective To investigate the effect of yiqihuoxue prescriptions on expression of COX-1 and iNOS after carotid artery vascular injury in rats. Methods Rats（n=90） were randomly divided into 5 groups： the model group, the aspirin group, the low-dose Yiqihuoxue group, the mid-dose Yiqihuoxue group and the high - dose Yiqihuoxue group. All of the rats fed up with hypereholesterolemic diet for 30 days,then the 2.0 F balloon catheter injury was performed through the right femoral artery into the left common carotid artery of rats ,the balloon was inflated and deflated 3 times.The rats were given drugs into stomach from 1 day before injury and to 5 days, 14 days, 28 days after injury. 6 rats from each group were euthanized by exsanguination at appropriate time point. The injuried left common carotid arteries were carefully removed and harvested for SABC staining .The expression of COX-1 and iNOS was observed by immunohistochemical technique. . Results The expression of COX-1 reached maximum after injury, and reduced obviously when given drugs, and the expression of COX-1 was significantly lower in drug-group at 5 d, 14d and 28d when compared with model group（P〈0.05）. In contrast ,the expression of iNOS was minimum after injury, then increased obviously when given drugs, and the expression of iNOS was significantly higher in drug-group when compared with model group（P〈0.05）. Conclusion Yiqihuoxue prescriptions reduce the expression of COX-1 and elevate the expression of iNOS, which maybe associate with inhibiting the formation of experimental thrombus and then effectively decrease restenosis after balloon injury by inhibiting production of TXA2 and dilating vessels.

关 键 词：血管形成术 经腔 经皮冠状动脉 益气活血 大鼠 环氧化酶-1 诱导型一氧化氮合酶 血栓 颈总动脉 

分 类 号：Q95-33[生物学—动物学] R543.4[医药卫生—心血管疾病]