虎头兰组培快繁技术体系研究  

Rapid propagation techniques of Cymbidium hookerianum Rchb

作　　者：文慧婷[1] 张翠玲[1] 

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533

出　　处：《广东农业科学》2009年第1期54-56,共3页Guangdong Agricultural Sciences

摘　　要：通过对虎头兰无胚乳种子无菌萌发过程中原球茎诱导、继代增殖、试管苗生根等不同发育时期的培养基配方筛选研究,初步建立了一套组培快繁技术体系,结果表明:虎头兰无胚乳种子在温度(25±2)℃、光照强度1 500～2 000 lx、光照时间12 h/d的条件下,较适宜的原球茎诱导培养基为MS+6-BA1.0 mg/L+NAA0.5 mg/L,适宜的增殖培养基为MS+6-BA2.0 mg/L+NAA0.5 mg/L,适宜的生根培养基为1/2MS+NAA1.0 mg/L。Based on the reseach of different mediums on fresh bulb inducement, mutiplication, and tube sprout process of no endosperm seeds sterile germination, we establish a rapid propagation system of Cymbidium hookerianum Rchb. The final results shows, the best culture medium of orchid fresh archibulb is MS＋6-BA1.0 mg/L＋ NAA0.5 mg/L while temprature （25±2）℃ and light 1 500-2 000 lx, 12 h/d in no endosperm seeds of Cymbidium hookerianum Rchb, the best medium of mutiplication is MS＋ 6-BA2.0 mg/L ＋NAA0.5 mg/L, and the best medium of rhizogencsis is 1/2MS＋NAA1.0 mg/L.

关 键 词：虎头兰 无胚乳种子 无菌萌发 组织培养 

分 类 号：S682.31[农业科学—观赏园艺]