鲁粤两省黄瓜花叶病毒辣椒分离物CP基因序列分析及亚组鉴定  

Sequence Analysis of CP Gene and Subgroup Identification of CMV from Pepper in Guangdong and Shandong

作　　者：孙秀东[1] 周淑梅[2] 雷建军[3] 曹必好[3] 陈国菊[3] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]山东农业大学生命科学院,山东泰安271018 [3]华南农业大学园艺学院,广东广州510642

出　　处：《华南农业大学学报》2009年第1期110-112,共3页Journal of South China Agricultural University

基　　金：广东省科技攻关项目(2006B20201028);广州市攻关项目(2005Z2-E0071);广东省农业厅项目

摘　　要：从广东和山东呈现花叶病症的辣椒上分离纯化得到2个黄瓜花叶病毒Cucumber mosaic virus(CMV)的分离物.利用RT-PCR技术克隆了病毒分离物的外壳蛋白(CP)基因.核苷酸序列测定表明,CMV-GDLJ和CMV-SDLJ 2个病毒分离物CP基因序列长度为657bp,编码218个氨基酸.2个病毒分离物的CP基因序列与CMV亚组Ⅰ的6个株系CP基因序列同源性均在90%以上,而与亚组Ⅱ的3个株系同源性均低于80%,据此将分离物CMV-GD和CMV-TA归属于CMV亚组Ⅰ.Two CMV isolates were obtained from the pepper with mosaic symptom in Guangdong and Shandong. The coat protein （CP） gene of the isolates was cloned by using RT-PCR technology. Sequencing results showed that the CP gene of the isolate had 657bp, encoding 218 amino acids. Comparison of the CP gene nucleotide sequences indicated that the CP gene nucleotide sequences of the isolate shared over 90% of homology with that of 6 strains belonging to CMV subgroup I , but less than 80% with 3 strains of subgroup Ⅱ. Based on the homology, it was concluded that the two isolates belong to subgroup I.

关 键 词：辣椒 黄瓜花叶病毒 亚组Ⅰ CP基因 序列分析 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]