水泡性口炎病毒2种血清型核衣壳蛋白的原核表达及生物信息学分析  

Prokaryotic Expression and Bioinformatics Analysis of the Nucleprotein Genes of Vesicular Stomatitis Virus

作　　者：曾彩云[1] 陈茹[1,2] 谢青梅[1] 马静云[1] 曹永长[3] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东出入境检验检疫局,广东广州510623 [3]中山大学生命科学学院,广东广州510275

出　　处：《华南农业大学学报》2009年第1期116-118,共3页Journal of South China Agricultural University

摘　　要：用RT-PCR方法从水泡性口炎病毒(vsv)扩增印第安纳(Indiana)和新泽西(New Jersey)血清型核蛋白N基因,分别克隆至pMD20-T载体进行序列鉴定及生物信息学分析.构建重组表达质粒VSV-IN-pBCX和VSV-NJ-pB-CX,并经SDS-PAGE和Western-blot鉴定表明2种血清型病毒核衣壳蛋白N基因在BL21(DE3)宿主菌中成功表达,重组蛋白相对分子质量约为82 000,并能够与各自对应血清型阳性血清反应.这表明2个重组蛋白具有良好的抗原性,可作为用于建立水泡性口炎血清学诊断方法的诊断抗原.The nucleoprotein genes of vesicular stomatitis virus （VSV, Indiana type and New Jersey type） were amplified by reverse transcription polymerase chain reaction （RT-PCR）, and then cloned into pMD20-T vector for sequencing and bioinformatics analysis. These genes were inserted into the prokaryotic expression plasmids pBCX. The recombinant plasmid VSV-IN-pBCX and VSV-NJ-pBCX were transformed into Escherichia. coli BL21 （DE3） cells. The relative molecular mass of the recombinant protein was 82 000, which was detected by SDS-PAGE and reacted with VS positive serum in Western-blot. The recombinant nucleoproteins can be used as the specific diagnosis antigens for immunoassay such as ELISA.

关 键 词：水泡性口炎病毒 核衣壳蛋白基因 原核表达 

分 类 号：S852.653[农业科学—基础兽医学]