滇重楼活性物质的分离鉴定与体外药理作用的研究  

Study on Separation,Identification and Pharmacological Activity of Paris Yunnanensis

作　　者：刘翊[1] 杜连祥[1] 高文远[2] 王旭[2] 高伟[1] 樊欣迎[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457 [2]天津大学药物科学与技术学院,天津300072

出　　处：《药物生物技术》2008年第6期481-484,共4页Pharmaceutical Biotechnology

摘　　要：研究滇重楼中甾体皂苷类化合物的体外抗肿瘤作用。采用硅胶柱层析、凝胶色谱、制备高效液相色谱(P-HPLC)及高效液相色谱(HPLC)等分离手段,对滇重楼各溶剂萃取部分进行了分离纯化,得到了7个甾体化合物。本实验在前期实验证明滇重楼乙酸乙酯和正丁醇提取物具有一定的抗肿瘤作用的基础上,以LA795小鼠肺腺癌细胞为研究对象,进一步考察了分离鉴定的单体化合物的抗肿瘤作用。结果表明,单体化合物1～7均表现出对LA795细胞生长的抑制作用;化合物1、2对LA795细胞具有明确的抑制作用,化合物3～7对LA795细胞具有较强的细胞毒活性。讨论了细胞毒活性与化合物结构之间的构效关系,即皂苷元C-3位连接糖链时,糖的数目及种类、连接方式及组成类型对其生物活性都起着至关重要的作用。To study the anti-tumor bioactive sis, column chromatography, Sephadex LH steroid saponins of Paris polyphylla Smith var. yunnanen compounds was obtained. Our previous study 20, preparative P-HPLC and HPLC were used and seven showed that the EtOAc and n-BuOH extracts revealed significant antitumor activities, and the further research for isolated compounds was screened by cytoxicity effects with LA795 cells. Compounds revealed certain inhibitory effect on LA795, especially compounds 1,2 and compounds 3～7 revealed significant inhibitory effect on LA795. Based on the results of pharmacological experiment, the number of glycosides and their linked position in the structure of saponins strongly influenced its antitumor activity.

关 键 词：滇重楼 甾体皂苷 细胞毒活性 

分 类 号：R28[医药卫生—中药学]