陕北白绒山羊HSL基因外显子1的SNPs及其与部分生产性状的相关分析  

Study on SNPs of exon 1 of HSL gene and its relationship with several production traits of Shaanbei white cashmere goats

作　　者：刘俊霞[1] 罗军[1] 滕炎玲[1] 绳贺军[1] 林先滋[1] 余刚[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第1期1-4,10,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：教育部新世纪优秀人才支持计划项目(NCET-05-0857)

摘　　要：【目的】探讨陕北白绒山羊激素敏感脂酶(Hormone sensitive lipase,HSL)基因外显子1的SNP遗传多态性及其与产绒、胴体和生长性状的相关关系,为陕北白绒山羊分子标记辅助选择提供理论依据。【方法】测定168只陕北白绒山羊部分生长、胴体指标。利用PCR-SSCP技术分析HSL基因外显子1的SNP遗传多态性。【结果】陕北白绒山羊HSL基因外显子1有AB和AA 2种基因型,在群体中处于Hardy-Weinberg平衡状态;AB与AA基因型个体的绒长差异极显著(P<0.01),体高和背膘厚差异显著(P<0.05),其他性状均无显著差异;AB基因型个体初生重、断奶重、绒长、周岁重、体高、体长、管围、背膘厚大于AA基因型,但AA基因型个体毛长、产绒量、胸围、眼肌面积大于AB基因型。【结论】陕北白绒山羊HSL基因外显子1具有多态性,该基因可能是陕北白绒山羊产绒及胴体性状的主效基因,或者与控制这些性状的主效基因相连锁。[Objective] This research analyzed the single nucleotide polymorphisms （SNPs） in the exon 1 of HSL gene and its relationship with some growth and carcass traits in Shaanbei White-Cashmere Goat in order to provide a theoretical foundation for the marker-assisted selection. [Method] Several growth and carcass traits of 168 Shaanbei white cashmere goats were measured in the study. PCR-SSCP technique was used to analyze the SNPs at the exon 1 of the HSL gene. [Result] The exon 1 of HSL gene displayed AA and AB genotypes and the population was at the Hardy-Weinberg equilibrium. There was a significant difference between the genotype AB and AA for cashmere length （P〈0.01）, wither height and back-fat thickness （P〈0.05）. There was no significant difference for other traits. The trend of ＂AB〉AA＂ was noted for birth weight, weaning weight, cashmere length, yearling weight, wither height, body length, circumference of cannon and back-fat thickness. The trend of ＂AA〉AB＂ was observed in the traits of hair length,cashmere yield,heart girth and loin eye area. [Conclusion] The exon 1 of HSL gene of Shaanbei white cashmere goat demonstrated polymorphism. It suggested that HSL gene might be a major gene or a major gene-linked gene for growth and carcass traits of Shaanbei white cashmere goats.

关 键 词：陕北白绒山羊 HSL基因外显子1 PCR-SSCP 单核苷酸多态性 

分 类 号：S827.2[农业科学—畜牧学]