弥散性神经内分泌细胞标志物在妊娠期山羊黄体细胞中的共存  

The coexistence of diffused neuroendocrine system cell markers in corpus luteum cells of pregency goat

作　　者：陈树林[1,2] 段会娟[1] 张宝[1] 雷治海[2] 蒋田园[1] 林磊[1] 李兆才[1] 马永生[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《西北农林科技大学学报（自然科学版）》2009年第1期22-28,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30170683);陕西省自然科学基金项目(2006C130)

摘　　要：【目的】探讨S-100蛋白、5-羟色胺（5-HT）、神经特异性烯醇化酶（NSE）和突触素（SYP）4种弥散性神经内分泌细胞标志物在黄体细胞中的共存情况。【方法】采取妊娠中期（50～70d）奶山羊卵巢，常规方法制备石蜡切片，采用免疫荧光双标记法结合激光扫描共聚焦显微镜观察，对4种弥散性神经内分泌细胞标志物的分布进行了检测。【结果】S-100蛋白与SYP共存于同一黄体细胞中，表达呈强阳性，阳性细胞主要分布在黄体周边，占总阳性细胞的56．57％。S-100蛋白与5-HT、5HT与SYP、NSE与SYP、5-HT与NSE、S-100蛋白与NSE共存的细胞呈弱阳性，分别占总阳性细胞的18．42％，15．67％，4．31％，4．30％和0．73％，均匀分布于整个黄体中。【结论】S-100蛋白、5HT、NSE和SYP在山羊黄体细胞中有两两共存现象。[Objective] The study investigated the coexistence of four diffused neuroendocrine markers in the corpus luteum cells, including S-100 protein, 5-hydroxytryptamine （5-HT）, neuron specific enolase （NSE） and synaptophysin （SYP）. [Method] Four diffused neuroendocrine markers in dairy goat ovary slice during mid-trimester of pregnancy（50-70 d） were determined by double immunofluorescence labeling for confocal laser scanning microscopy. [Result] Coexpression of S-100 protein and SYP expressed in the same corpus luteum cell and expression was strongly positive,which distributed in the perimeter of the corpus luteum. The positive cells coexpressing S-100 protein and SYP accounted for 56.57% of the total positive cells. Coexistence of S-100 protein and 5-HT, 5-HT and SYP, NSE and SYP, 5-HT and NSE, S-100 protein and NSE were weakly positive. They accounted for 18.42%,15.67%,4.31%,4.30% and 0.73% of the total positive cells respectively, and distributed uniformly in corpus luteum. [Conclusion] Coexistence between four diffused neuroendocrine markers in the corpus luteum cells of dairy goat was presented.

关 键 词：神经内分泌标志物 免疫荧光双标记 激光扫描共聚焦显微镜 黄体 山羊 

分 类 号：S827.1[农业科学—畜牧学]