新城疫病毒分离株P基因的分子特性和遗传进化  

Genetic characteristics and evolution of phosphoprotein gene of the field Newcastle disease virus

作　　者：梁俊文[1,2] 田夫林[3] 黄兵[4] 兰邹然[3] 孙圣福[3] 庄文忠[1] 

机构地区：[1]山东师范大学生命科学院,山东济南250014 [2]山东省动物卫生监督所,山东济南250022 [3]山东省动物疫病预防与控制中心,山东济南250022 [4]山东省农业科学院家禽研究所,山东济南250023

出　　处：《西北农林科技大学学报（自然科学版）》2009年第1期49-55,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：山东省科技攻关项目(031020101)

摘　　要：【目的】探讨新城疫病毒（Newcastle disease virus，NDV）磷蛋白基因（P基因）的分子进化特点，为科学防控新城疫（Newcastle disease，ND）提供依据。【方法】对1997～2007年国内分离的13个NDV毒株的P基因进行扩增测序，与15个已发表的国内外不同时期的NDV毒株P基因进行核苷酸和推导的氨基酸遗传变异分析及分子特性研究。【结果】国内NDV分离株P基因核苷酸和P蛋白氨基酸高度同源，同源性分别为93．9％～99．8％和93．4％～99．2％。国内NDV毒株与LaSota、Clone30和F48E9等P基因核苷酸和P蛋白氨基酸同源性分别为82．2％～87．7％，81．3％～83．8％；与国外毒株则分别为82．1％～90．2％，81．1％～90．7％。氨基酸序列分析表明，我国毒株具有独特的氨基酸位点。分子进化分析表明，NDVP基因中核苷酸同义替代率高于非同义替代率。【结论】国内分离NDV毒株P蛋白遗传距离较近，而与LaSota、Clone30、F48E9以及国外毒株遗传距离较远，有一定的地域性。NDVP基因以净化选择的方式进化。[Objective] To scientifically control and prevent Newcastle disease （ND）, this study researched the molecular evolution characteristics of Phosphoprotein（P） gene of Newcastle disease viruses （NDV）. [Method] The nucleotide sequences and deduced amino acid sequences were analyzed for P gene of thirteen field isolates of NDV during 1997-2007 in China. The P gene sequences of fifteen NDV reference strains in GenBank were also used in this study. [Result] The nucleotide and amino acid homology of Chinese field NDV strains was 93.9%-99.8% and 93.4%-99.2% respectively,but 82.2%-87.7% and 81.3%- 83.8% compared with LaSota,Clone30 and F48E9,and 82.1%-90.2% and 81.1%-90.7% to foreign NDV strains. The unique residues in the amino acid sequence were found in the Chinese isolates. Synonymous nucleotide substitutions were more than non-synonymous substitutions. [Conclusion] There was the nearest distance, compared with the LaSota, Clone30 and F48Eg, and the character of geographical areas among Chinese NDV isolates. The evolution of NDV P gene underwent purifying selection.

关 键 词：新城疫病毒 P蛋白 P基因 

分 类 号：S852.659.5[农业科学—基础兽医学]