人源抗菌肽LL-37原核表达载体的构建及表达  

Construction of Human Antimicrobial Peptide LL-37 Prokaryotic Expression Vector and Its Expression

作　　者：顾银霞[1] 周学章[1] 宋振威[1] 李敏[1] 王玉炯[1] 

机构地区：[1]宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021

出　　处：《宁夏大学学报（自然科学版）》2008年第4期337-339,347,共4页Journal of Ningxia University(Natural Science Edition)

基　　金：国家"973"计划基金资助项目(2006CB504401)

摘　　要：为了使人源抗菌肽LL-37在原核表达载体中获得高效表达,在不改变LL-37氨基酸的基础上,根据大肠杆菌偏爱密码子表,替换LL-37部分密码子,设计一段新的LL-37序列.采用DNA Work设计4条互补引物,利用SOE-PCR技术扩增完整的LL-37目的片段,以pET-PPPI为载体,构建原核表达载体pET-PPPIL,并制备表达菌BL21(DE3)(pET-PPPIL),该菌经诱导高效表达LL-37融合蛋白,为后续纯化LL-37奠定了基础.To make highly expressed human antimicrobial peptide LL-37 in prokaryotic expression system, the part of codon of LL-37 was replaced and a new LL-37 series was designed in this work. Four complementary primers were designed by DNA Work, the integrity of the LL-37 fragment was amplified by SOE-PCR, then expression vector pET-PPPIL was constructed with pET-PPPI as the carrier, and prepared expression strain BL21 （DE3） （pET-PPPIL） which is induced expresses LL-37 fusion protein highly, this results lay a foundation for the purifing LL-37.

关 键 词：聚合酶链式反应 人源抗菌肽LL-37 蛋白表达 

分 类 号：Q789[生物学—分子生物学]