高效液相色谱法测定盐酸消旋山莨菪碱注射液中消旋山莨菪碱的含量  

Determination of raceanisodamine hydrochloride injection by HPLC

作　　者：陈敏 

机构地区：[1]福建省福州市药品检验所,福州350007

出　　处：《海峡药学》2008年第12期37-39,共3页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定盐酸消旋山莨菪碱注射液的含量。方法采用Hypersil ODS2柱(4.6mm×250mm,5μm),流动相为0.02%三乙胺溶液-甲醇(50∶50),检测波长为220nm,流速为1.0mL.min-1。结果消旋山莨菪碱进样量1.065μg^6.390μg与峰面积呈良好线性关系(r=0.9999),平均回收率为100.10%,RSD为0.85%(n=9)。结论该方法操作简便、结果准确、重现性好。OBJECTIVE To establish a method for determining the contents of raceanisodamine in raceanisodamine hydrochloride injection by HPLC. METHODS The chromatographic column was Hypersil ODS2 ; the mobile phase was 0.02 % trienthylamine-methanol （50 ： 50） ; the flow rate was 1.0mL· min^- 1 and the detection wavelength was 220nm.RESULTS The linear range of raceanisodamine was from 1.065μg--6.390μg （r = 0. 9999）. The average recovery of sample was 100.10 % and RSD was 0. 85 % （n = 9）. CONCLUSION This method is simple and rapid and accurate. It can be effectively used for the quality control of Raceanisodamine Hydrochloride Injection.

关 键 词：盐酸消旋山莨菪碱注射液 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]