棉铃虫抗辛硫磷品系的代谢抗性机理  

Metabolic Resistance Mechanisms in a Phoxim-resistant Strain of Helicoverpa armigera

作　　者：郑央萍[1,2] 杨亦桦[1] 吴益东[1] 

机构地区：[1]南京农业大学植物保护学院昆虫学系／农业部作物病虫监测与防控重点开放实验室,南京210095 [2]环境保护部南京环境科学研究所,南京210042

出　　处：《农药学学报》2008年第4期410-416,共7页Chinese Journal of Pesticide Science

基　　金：商品共同基金(CFC/ICAC-14)

摘　　要：通过重复回交和药剂选择,将棉铃虫Phoxim-R抗性品系对辛硫磷的抗性导入到BK77敏感品系中,得到棉铃虫BK77-R抗性品系,BK77-R和BK77为一对近等基因系。BK77-R抗性品系对辛硫磷的抗性达155倍,对溴氰菊酯有高水平交互抗性(抗性倍数248倍),对灭多威和硫丹有中等水平交互抗性,分别为31倍和11倍,对丙溴磷有低水平交互抗性(4倍)。在BK77-R抗性品系中,脱叶磷(DEF,酯酶抑制剂)对辛硫磷、灭多威和硫丹具有增效作用,增效倍数分别为7倍、2倍和1.9倍;增效醚(PBO,氧化酶抑制剂)对溴氰菊酯、灭多威和辛硫磷的增效倍数分别为21倍、2.2倍和1.7倍。与BK77敏感品系相比,BK77-R抗性品系的酯酶和多功能氧化酶活性均显著提高,而谷胱甘肽S-转移酶活性没有明显变化。上述结果表明,酯酶解毒代谢在棉铃虫BK77-R品系对辛硫磷的抗性中起重要作用,酯酶和多功能氧化酶解毒作用增强是该抗性品系对不同类型药剂产生交互抗性的重要原因。Phoxim resistance in a resistant Phoxim-R strain of Helicoverpa armigera was introgressed into a susceptible BK77 strain through repeated backcrossing and selection with phoxim. The introgressed strain （BK77-R） and the BK77 strain were a pair of near-isogenic strains. The BK77-R strain not only developed 155-fold resistance to phoxim compared with the BK77, but it also obtained high level cross resistance to deltamethrin （ resistance factor, RF 248-fold）, middle level cross resistance to methomyl （RF 31-fold） and endosulfan （RF 11-fold）, and low level cross resistance to profenofos （ RF 4-fold）. S, S, S-tributylphosphorotrithioate （ DEF, esterase inhibitor） had significant synergism to phoxim （ synergism ratio, SR 7-fold ）, methomyl （ SR 2-fold ） and endosulfan （ SR 1.9-fold）. Piperonyl butoxide （PBO, oxidase inhibitor） had synergistic effect against deltamethrin （SR 21 n-fold）, methomyl （SR 2.2-fold） and phoxim （SR 1.7-fold）. The oxidase and esterase activity of the BK77-R strain was significantly higher than that of the susceptible BK77 strain, and the glutathione S-transferase activity was not significantly altered. The results demonstrated that esterase-mediated metabolism plays an important role in pboxim resistance in the BK77-R strain, and enhanced detoxification mediated by both esterases and oxidases is an important factor conferring broad cross-resistance to different classes of insecticides.

关 键 词：棉铃虫 辛硫磷 代谢抗性 酯酶 多功能氧化酶 

分 类 号：S481.4[农业科学—农药学]