血管紧张素-(1-7)对THP-1源性巨噬细胞CD40/CD40L表达的影响  

The effect of angiotensin-(1-7) on expression of CD40/CD40L in THP-1 cells

作　　者：张利峰[1] 杨志明[2] 柴蝉娟[1] 吴蕊[1] 

机构地区：[1]山西医科大学实验中心,太原市030001 [2]山西医科大学附属第二医院

出　　处：《中国心血管病研究》2008年第10期768-770,共3页Chinese Journal of Cardiovascular Research

摘　　要：目的探讨血管紧张素-(1-7)[Ang-(1-7)]对血管紧张素Ⅱ[AngⅡ]刺激下THP-1源性巨噬细胞CD40和CD40L表达的影响。方法用一定浓度的AngⅡ诱导体外培养的巨噬细胞,与不同浓度的Ang-(1-7)共同孵育,应用流式细胞技术检测CD40和CD40L在细胞上的表达,采用反转录-聚合酶链反应(RT-PCR)检测CD40和CD40LmRNA表达。结果Ang-(1-7)既可下调细胞表面CD40和CD40L的表达,又能使CD40和CD40LmRNA表达下降,其阻断作用呈浓度依赖性;应用Ang-(1-7)特异性阻断剂A-779后CD40和CD40L表达再次升高,与AngⅡ组比较差异无统计学意义(P>0.05)。结论Ang-(1-7)可拮抗巨噬细胞CD40和CD40L表达,并呈浓度依赖性,这种作用可能是其抗动脉粥样硬化的机制之一;Ang-(1-7)是通过特异性受体MAS起作用的。Objective To investigate the effects of angiotensin （1-7）[Ang-（1-7）] on expression of CD40,CD40L in THP-1 cells,which is stimulated by angiotensin Ⅱ （Ang Ⅱ ）, identifying the protection of Ang-（ 1- 7） in cardiopathy.Methods THP- 1 ceils were induced by fixed concentration of Ang Ⅱ and different concentration of Ang-（1-7）, the CD40,CD40L expression on the macrophage were detected by flow cytometric analysis and CD40, CD40L mRNA were detected by RT-PCR.Results Ang-（1-7） can not only down-regulated the expression of CD40 and CD40L, but also the mRNA levels of CD40 and CD40L in cultured macrophage, in addition the Ang-（1-7） inhibitied them concentration dependently, the expression of CD40,CD40L step up again after using A-779 which is a specified blokiag agent of Ang-（ 1-7）,and it is similar to the group of Ang Ⅱ. Conclusion Ang-（ 1-7 ） inhibited the expression of CD40, CD40L dependently in THP-1 cells,which is stimulated by Ang Ⅱ , the antagonism of Ang-（ 1-7 ） on the expression of CD40, CD40L is related to its specified acceptor-MAS.

关 键 词：血管紧张素-(1—7) 血管紧张素II CD40/CD40L通路 THP-1巨噬细胞 

分 类 号：Q95-33[生物学—动物学] R541.4[医药卫生—心血管疾病]