吸烟患者尿液中一氧化氮与尼古丁代谢产物-柯替宁含量的相关性研究  

The correlative study on nitric oxide and cotinine nicotine metabolite in smokers′urine

作　　者：庞宝森[1] 翁心植[1] 王辰[1] 柯以恕[1] 张红玉 张海燕[1] 黄秀霞[1] 赵离忠 

机构地区：[1]北京红十字朝阳医院呼吸病研究中心

出　　处：《中华结核和呼吸杂志》1998年第4期215-217,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的观察吸烟对体内一氧化氮（ＮＯ）含量变化的影响及了解它们之间的关系。方法选择北京地区健康６０名男性不吸烟者（不吸烟组）及１９２名男性吸烟者，按其每日吸烟支数分为１～１０支组、１１～２０支组、２１～３０支组、＞３０支组。用硫代巴比妥酸法测定吸烟者尿液中尼古丁代谢产物柯替宁（ｃｏｔｉｎｉｎｓ）作为吸烟量的客观指标，用分光比色法测定尿中ＮＯ含量，来反映体内ＮＯ水平。结果吸烟者各组尿液中柯替宁含量分别为６．６±１．６μｍｏｌ／Ｌ、２５±６μｍｏｌ／Ｌ、３７±９ｍｏｌ／Ｌ、５４±８μｍｏｌ／Ｌ。不吸烟者尿液中ＮＯ含量为４．４±１．０μｍｏｌ／Ｌ，吸烟者各组尿液中ＮＯ含量分别为３．０４±０．０７μｍｏｌ／Ｌ、２．７５±０．０５μｍｏｌ／Ｌ、２．１８±０．０１μｍｏｌ／Ｌ、１．６０±０．０１μｍｏｌ／Ｌ。吸烟者尿液中柯替宁含量各组间依次分别为ｔ＝４．２８９４，Ｐ＜０．０１；ｔ＝２．７４４６、Ｐ＜０．０１；ｔ＝２．７６６７、Ｐ＜０．０１。吸烟者各组尿液中ＮＯ含量与不吸烟者组比较分别为ｔ＝２．７１１８、Ｐ＜０．０１；ｔ＝３．１８２６、Ｐ＜０．０１；ｔ＝３．２１００、Ｐ＜０．０１；ｔ＝４．６６００、Ｐ＜０．０１。尿液中ＮＯ?Objective In order to observe the changes of nitric oxide (NO) affected by smoking and their relationship in vivo. Method 60 male nonsmoker as the control group, 192 male smoked as the study group which was divided into 4 subgroups (1～10, 11～20, 21～30 and >30) according to the number of cigarettes smoked per day. The metabolite of nicotine cotinine in urine was assayed by means of barbituric acid. Cotinine was used as an objective indicator to reflect the smoking quantity. The urinary NO (NO - 2) was determined with colorimetric estimation. Result The urinary cotinine level in the 4 subgroups were 6.6±1.6, 25.1±6.2, 37.8± 8.5, 53.6±8.3 (μmol/L) respectively. The urinary NO of nonsmokers were 4.43±0.97 (μmol/L), in the 4 subgroups it was 3.04±0.07, 2.75± 0.05, 2.18±0.01, 1.60±0.01 (μmol/L) respectively. The results of statistical analysis between the 4 subgroups were t =4.2894, P <0.01; t =2.7446, P <0.01; t =2.7667, P <0.01 acordingly. As compared between the 4 subgoups of smokers and the nonsmokers, the statistical results were t = 2.7118, P <0.01; t =3.1826, P <0.01; t =3.2100, P <0.01; t =4.6600, P <0.01; respctively. The statistical differences between the 4 subgroups were t =2.9286, P <0.01; t =3.0357, P <0.01; t =3.8181, P <0.01; t =2.2160, P <0.05 respectively. There was a good positive correlation between the number of cigarette smoked and urinary cotinine content ( r =0.8626, P <0.01), also a significant negative correlation between it and urinary NO level ( r =-0.6596, P <0.05). A negative correlation could be observed between urinary cotinine and NO ( r =-0.6026, P <0.05). Conclusion With the increase of quantity and duration of smoking, the NO content in vivo will decrease, suggested that the vascular endothelial cells could be damaged severely.

关 键 词：吸烟 一氧化氮 柯替宁 尿液 尼古丁 

分 类 号：R163[医药卫生—公共卫生与预防医学]