枸杞黄酮类化合物清除氧自由基及对小鼠L_(1210)癌细胞能量代谢的抑制作用  

Scavenging Effect of Total Flavonoids of Lycium Barbarum L Active Oxygen Radicals and Inhibitory Effects on Heat Output from L1210 Cells

作　　者：黄元庆[1,2] 谭安民[1,2] 沈泳[1,2] 卢景芬 刘宏宇 王洁[1,2] 

机构地区：[1]宁夏医学院预防医学系营养与食品卫生教研室 [2]北京医科大学天然药物与仿生药物国家重点实验室

出　　处：《卫生研究》1998年第2期109-111,115,共4页Journal of Hygiene Research

基　　金：国家自然科学基金;天然药物与仿生药物国家重点实验室基金

摘　　要：采用ＥＳＲ自旋捕捉法和微量量热法能量研究了枸杞总黄酮（ＴＦＬ）对活性氧自由基的清除作用，及对Ｌ１２１０细胞热代谢的影响。结果表明：在Ｆｅｎｔｏｎ体系中，ＴＦＬ７．５～２００ｍｇ／Ｌ对ＯＨ·的清除率为２０％～７２％；在Ｘａｎ／ＸＯ体系中ＴＦＬ０～２１７ｍｇ／Ｌ，Ｏ－·２清除率为０～５１％；ＴＦＬ０．５６ｇ／Ｌ对多形核白细胞ＰＭＮ呼吸爆发的产热峰完全抑制，对Ｌ１２１０细胞的能量代谢有明显抑制作用，对正常大鼠ＰＭＮ能量代谢有促进作用。The scavenging effects of total flavonoids of Lycium barbarum L.(TFL) were studied by using ESR spin trapping technique and the inhibitory effects on heat output of both polymorphonuclear leukocyte(PMN) respiration burst and L1210 cells were measured by suing microcalorimetric technique.TFL(0～217mg/L)could scavenge O-·2 in xanthine/xanthine oxidase (Xan/XO)system,with scavenging rate of 0～51%.TFL(75～200mg/L)could scavenge OH· produced in Fenton reaction and the scavenging rate is between 20% to 72%.Those effects were concentration dependent.Furthermore,TFL(056g/L)could completely inhibit the heat output from PMA stimulated PMN and TFL(10～50g/L)could inhibit the heat output from L1210 cells.

关 键 词：枸杞总黄酮 氧自由基 微量量热法 L1210细胞 

分 类 号：R285.5[医药卫生—中药学]