LDL对牛精子冷冻—解冻后运动特性的影响  

Effects of LDL on Movement Characteristic of Bovine Frozen Sperm

作　　者：崔山[1] 尹瑞卿[1] 张鹏[1] 胡建宏[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国牛业科学》2008年第6期8-12,共5页China Cattle Science

基　　金：西北农区生态型奶业生产技术和奶牛性控技术研究与开发(2006BAD04A11);国家"十一五"科技支撑计划

摘　　要：在牛冷冻精液稀释液中,卵黄中的低密度脂蛋白(LDL)对精子起主要保护作用。本研究利用含有7%、8%、9%和10%(w/v)LDL配制成的稀释液制作牛细管冷冻精液(0.25mL/支),冷冻-解冻后,通过计算机辅助分析系统(CASA)对精子运动参数进行分析。结果表明:稀释液中含8%(w/v)LDL时冷冻效果最好,其冻后精子活力平均可达55.6%,显著高于其他组(P<0.01);精子直线速度平均为32.5μm/s,平均路径速度为35.7μm/s,与对照组相比差异显著(P<0.05);a,b级精子可占精子总数的54%。本研究为牛冷冻精液新型稀释液的开发与利用提供了一定的依据。In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders. The protective action of yolk is largely presumed to be due to low density lipoprotein （LDL）. The objective of the present study was to obtain information about the cryoprotective effect of different concentrations LDL （7 %, 8 %, 9 %, 10 %, w/v） in bovine semen extender instead of egg yolk in 0.25 ml straws. The characteristics of sperm motion after frozen-thawed were evaluated with a computer-aided sperm analysis （CASA） system （Weili Software Co. Ltd, Beijing, China）. The results showed that the sperm motion characteristics were improved with LDL in the extender, as compared to the extender containing 20% yolk. The highest sperm motility was obtained with 8% LDL （w/v） and it was 55. 6%, which was significantly higher than that of other groups（ P 〈 0.01）. The straight line velocity reached 32. 5μm/s, the velocity of the average path was about 35.7μm/s, and they were significantly higher with semen frozen in 8% LDL extender compared to control group （ P 〈 0.05）. The percentage of the sperm of grade a and b was 50%. This study affords some way to the exploitation and utilize of bovine frozen sperm.

关 键 词：低密度脂蛋白 牛冷冻精液 精子运动性能 计算机辅助分析系统 

分 类 号：S823.3[农业科学—畜牧学]