腹透液相关浓度葡萄糖对人腹膜间皮细胞周期蛋白的影响及葛根素的拮抗作用  

Effect of glucose related to peritoneal dialysate on the cell cycle and protein p21 expression in human peritoneal mesothelial cells and the antagonism of puerarin

作　　者：曹东维[1] 张苗[1] 蒋春明[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肾科,江苏南京210008

出　　处：《徐州医学院学报》2008年第12期787-789,共3页Acta Academiae Medicinae Xuzhou

基　　金：南京市医学科技发展重点项目(ZKX0416)

摘　　要：目的研究腹透液相关浓度葡萄糖(1.5%、2.5%)对人腹膜间皮细胞周期和周期蛋白p21的影响及葛根素的拮抗作用。方法人腹膜间皮细胞在含糖(1.5%、2.5%)、含糖含葛根素(葛根素终浓度为100 mg/L)的无血清培养基中培养24 h后,用流式细胞仪分析细胞周期,Western blot测定p21蛋白的表达。结果经含糖1.5%和含糖2.5%培养基培养24 h后人腹膜间皮细胞大多出现肥大和停滞于细胞周期的G1期。高浓度葡萄糖刺激p21蛋白表达,含糖2.5%组和含糖1.5%组无显著性差异(P>0.05)。含葛根素含糖组腹膜间皮细胞中处于G1期细胞数较相应浓度含糖组减少,p21表达减少(P<0.05)。结论高糖可刺激人腹膜间皮细胞p21蛋白的表达,且可能与高糖作用下细胞肥大及G1期阻滞有关,葛根素可在一定程度上拮抗高糖对人腹膜间皮细胞周期和周期蛋白的影响。Objective To investigate the effect of glucose at different concentrations on the cell cycle and p21 expression in cultured human peritoneal mesothelial cells（HPMC） and protection of puerarin.Methods HPMCs were cultured with serum free RPMI 1640 medium containing glucose of different concentrations（1.5% and 2.5%） and puerarin（100 mg/L） for 24 hours.The cell cycle distribution was then investigated by flow cytometry.The expression of p21 was detected by Western blot.Results In the presence of 1.5% and 2.5% glucose concentrations,most of the cells became hypertrophic,and were arrested in G1 phase of cell cycle.High glucose stimulated expression of p21,which had no difference between the 1.5% and 2.5%（P〉0.05）.The number of the cells arrested in G1 and p21 expressions decreased in groups of puerarin.Conclusion High glucose stimulated the expression of p21,which might be related to the hypertrophy and arrest in the G1 phase of mesothelial cells by high glucose.Puerarin might antagonize the effect of high glucose of the cycles of mesothelial cells and their cyclin.

关 键 词：腹膜间皮细胞 葡萄糖 细胞周期蛋白 葛根素 

分 类 号：R692.5[医药卫生—泌尿科学]