激活PPARα对海马神经元胆固醇外流的影响  

Effect of PPARα activation on cholesterol efflux in hippocampal neuron

作　　者：张淑坤[1] 姜玲玲[1] 刘赫[1] 石芸[1] 侯艳宁[2] 

机构地区：[1]河北医科大学生物化学与分子生物学教研室,河北石家庄050017 [2]白求恩国际和平医院

出　　处：《中国老年学杂志》2009年第1期6-8,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(30371566);河北省自然科学基金(303472)资助

摘　　要：目的探讨激活过氧化物酶体增殖物激活受体α(peroxisome proliferators-activated receptorsα,PPARα)对海马神经元胆固醇外流的影响及可能机制。方法在大鼠海马神经元培养液中加入0.1mmol/L氯贝丁酯,用RT-PCR方法观察肝X受体(liver X receptor,LXR)及其靶基因ATP结合盒转运体A1(ATP binding cassette transporter A1,ABCA1)mRNA表达,用胆固醇氧化酶-比色法检测细胞胆固醇排出量的变化。结果氯贝丁酯降低海马神经元LXR和ABCA1mRNA表达(P<0.05),降低胆固醇排出量(P<0.05)。结论激活PPARα可能通过抑制LXR-ABCA1途径减少海马神经元胆固醇外流。Objective To investigate the effect of peroxisome proliferators-activated receptors a（PPARα） activation on cholesterol efflux in hippocampal neurons. Methods Hippocampal neurons were treated with 0. 1 mmol/L clofibrate and dissolved in dimethylsulfoxide for 48 h. The expression of liver X receptor （LXR） mRNA and its target gene ATP binding cassette transporter A1 （ ABCA1 ） mRNA were measured by RT-PCR. Cholesterol efflux of neuron was determinated by enzymatic-colorimetric assay. Results The expression of LXR mRNA and ABCA1 mRNA were reduced and cholesterol efflux was significantly decreased in clofibrate-treated hippocampal neurons. Conclusions PPARα activation may be through inhibiting the LXR-ABCA1 pathway to reduce cholesterol efflux in hippocampal neurons.

关 键 词：过氧化物酶体增殖物激活受体 氯贝丁酯 肝X受体 ATP结合盒转运体A1 胆固醇外流 

分 类 号：Q189[生物学—神经生物学]