青蒿琥酯钠对兔耳增生性瘢痕中肥大细胞的影响  

Inhibiting effect of artesunate on mastocyte in rabbit ear hypertrophic scar

作　　者：李伟[1] 石崇荣[2] 黄崇本[2] 吴一[1] 

机构地区：[1]重庆市急救医疗中心整形科,重庆400014 [2]重庆医科大学附属第一医院烧伤整形科,重庆400016

出　　处：《中国美容医学》2009年第1期66-68,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨青蒿琥酯钠(Art)对兔耳增生性瘢痕中肥大细胞的影响。方法:用新西兰大白兔制作增生性瘢痕动物模型,兔左耳为Art组,用60mg/mlArt20μl/瘢痕作局部注射,每隔2天1次,共5次;右耳作为对照组,用5%NaHCO320μl/瘢痕作局部注射。HE、VG染色观察成纤维细胞和胶原形态,甲苯胺蓝染色作肥大细胞计数。结果:HE和VG染色显示:Art组真皮层较薄,胶原纤维排列较整齐;对照组真皮层较厚,胶原排列紊乱,有漩涡状结构。肥大细胞计数Art组为(5.27±1.27)个/HP,对照组为(10.67±2.35)个/HP,两组比较差异有显著性意义。结论:青蒿琥酯钠能减少瘢痕中肥大细胞数量,抑制成纤维细胞增殖,减少胶原合成。Objective To probe into the inhibiting effect of Artesunate on Mastocyte in rabbit ear hypertrophic scar model. Methods Six New Zealand rabbits were used to establish animal model for hypertrophic scar. The scars on the left ears were taken as Artesunate group receiving sodium Artesunate by local injection in a dose of 60mg/ml, 20μl pre scar every two days for 15 days. The scars on the right ears served as control group were treated with 5% sodium bicarbonate by local injection 20μl pre scar. After 15 days of treatment, the scars were harvested. HE and VG staining were used to observe the histomorphology of the fibroblasts and collagen fibers. Mastocyte were counted on Toluidine blue staining slide. Results On gross examination,the hardness of the scars of the Artesunate group was lower than that of control group. The dermis of the Artesunate group was thinner than that of control group, the collagen was well-arranged in Artesunate group. HE and VG staining shown more fibroblasts and the collagen nodules could be found in control group. There was significant difference between the two groups in mastocyte counts （P〈0.01）.The mastocyte count of Artesunate group and control group were （5.27±1.27）/HP and （10.67±2.35）/HP respectively. Conclusions It shows Artesunate can inhibit fibroblast proliferation by reducing mastocyte and reduce collagen synthesis.

关 键 词：青蒿琥酯钠 动物模型 增生性瘢痕 肥大细胞 

分 类 号：Q813.1[生物学—生物工程]