维生素E与地塞米松对小鼠急性肺损伤炎症及肌球蛋白轻链激酶表达的影响  

The effects of vitamin E and dexamethasone on inflammation of acute lung injury and expression of myosin light chain kinase

作　　者：白建文[1] 邓伟吾[2] 林闽加[1] 许淑敏[1] 张斗霞[1] 

机构地区：[1]同济大学附属东方医院急诊医学部,上海200120 [2]上海交通大学附属瑞金医院呼吸科

出　　处：《中华结核和呼吸杂志》2009年第1期46-50,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：上海市自然科学基金资助项目（03ZR14078）

摘　　要：目的观察维生素E联合地塞米松对小鼠急性肺损伤炎症及肌球蛋白轻链激酶（MLCK）表达的影响。方法将40只8周龄Ba1b／c雌性小鼠按随机数字表法分为4组：生理盐水对照组（1．5ml／kg）、细菌脂多糖组（1mg／kg）、维生素E联合地塞米松治疗组（联合治疗组）、维生素E治疗组（维生素E组）。联合治疗组给予腹腔注射维生素E 50mg／kg和地塞米松1mg/kg，1h后再给予脂多糖1mg／kg滴鼻，维生素E组仅给予维生素E脂质体50mg／kg，观察各组小鼠肺组织病理改变。采用免疫组织化学SABC法检测肺组织MLCK免疫反应细胞，用逆转录-PCR反应榆测肺组织中MLCK mRNA的表达，Western blot法检测肺组织中MLCK蛋白的表达。各组间均数比较采用单因素方差分析，均数两两比较采用SNK检验。结果脂多糖组小鼠的肺部炎症反应显著，以中性粒细胞浸润为主，伴明显的肺泡充血、水肿。两治疗组肺组织炎症、充血明显改善；对照组、脂多糖组、联合治疗组、维生素E组BALF中细胞总数分别为（1．1±0．4）、（5．6±2．1）、（4．0±1．0）、（4．2±1．3）×10^９/L，脂多糖组与其他3组比较，差异有统计学意义（F=14．53，均P〈0．05）。脂多糖组MLCK免疫反应细胞较对照组明显增加，主要在上皮和内皮，联合治疗组和维生素E组MLCK免疫反应细胞明显减少；脂多糖组与两治疗组肺组织MLCK mRNA吸光度值比较，差异无统计学意义（F=2．76，均P〉0．05）；脂多糖组与两治疗组肺组织MLCK蛋白吸光度值比较，差异有统计学意义（F=12．06，均P〈0．01）。结论维生素E联合地塞米松可抑制脂多糖诱导的小鼠急性肺损伤肺部炎症及肺组织MLCK蛋白的表达，其作用可能部分通过抑制MLCK活性而达到稳定血管屏障、改善肺水肿和炎症。Objective To observe the effect of vitamin E （VitE） combined with dexamethasone （DXM） on inflammation of acute lung injury and expression of myosin light chain kinase. Methods Forty female Balb/c mice were randomly divided into 4 groups, a saline control group（ 1.5 ml/kg） , a LPS group （1 mg/kg）, a VitE and DXM group（VitE 50 mg/kg, DXM 1 mg/kg）, and a VitE group（50 mg/kg）. Lung tissue histopathological changes were observed. Immunohistochemistry assays （SABC）were used to determine the myosin light chain kinase（MLCK） immunoreaetive cells in the lung tissues, and the MLCK mRNA and the MLCK protein was assayed by RT-PCR and by Western blot, respectively. Means were compared with analysis of variance and Student-Newman-Keuls were used to compare 2 means. Results Histological examination showed that extensive lung inflammation were seen in the LPS group, which manifested by accumulation of significant numbers of neutrophils, accompanied by marked pulmonary edema and hemorrhage. The inflammation and hemorrhage in the 2 treatment groups were significantly improved. Immunoreactive cells of MLCK numbers in BALF in the control group, the LPS group, the VitE and DXM group, and the VitE group was （1.1±0.4）, （5.6±2.1）, （4.0±1.0）, （4.2±1.3）× 10^9/L respectively. Compared with other groups, the difference of LPS group was significant （ F = 14. 53, all P 〈 0. 05 ）. hnmunoreactive cells of MLCK located airway epithelial and endothelia in the LPS group were more than which in the control group, decreased immunoreaetive cells of MLCK in two treatment groups. Compared with LPS group, the difference of MLCK mRNA expression （A） of lung tissue in two treatment groups was no significant（F = 2. 76,all P 〉0. 05）. Compared with LPS group, the difference of A values of MLCK protein of lung tissue in two treatment groups was statistical significance （F=12. 06 ,all P 〈 0. 01 ）. Conclusions Vitamin E combined with low dose of DXM could effectively inhibit inflammation a

关 键 词：维生素E 地塞米松 肌球蛋白轻链激酶 呼吸窘迫综合征 成人 

分 类 号：R686[医药卫生—骨科学]