枸杞多糖对大鼠心室肌细胞L型钙电流的影响  

Effects of Lycium Barbarum Polysaccharides on L-Type Calcium Channel Current in Rat Ventricular Myocytes

作　　者：刘淼[1] 唐其柱[1] 王晓玲[1] 汪晶晶[1] 王腾[1] 

机构地区：[1]武汉大学人民医院心血管病研究所,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2009年第1期57-60,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金资助项目(编号:301130565)

摘　　要：目的:探讨枸杞多糖对大鼠心室肌细胞L-型钙通道的作用。方法:用急性酶解法获得大鼠的单个心肌细胞,用标准的全细胞膜片钳技术记录钙通道电流。结果:①枸杞多糖能使I-V曲线明显上移。②枸杞多糖对ICa-L呈浓度依赖性的抑制作用。③50 mg/L枸杞多糖能使ICa-L稳态激活曲线右移、还能增加失活后恢复的τ值;但对失活曲线无影响,不改变ICa-L失活特性。结论:枸杞多糖对ICa-L有阻断作用,主要作用于钙通道激活和恢复过程。Objective： To study the effects of Lycium Barbarum polysaccharides（LBP） on L type calcium channel current （ICa-L） in rat ventricular myocytes. Methods： Standard whole-cell patch clamp technique was used. Results： （1）LBP could make the I V curves upward. （2）LBP reduced the ICa-L in a concentration-dependent manner. （3） LBP in the concentration of 50 mg/L made the steady state activation curves shift to right, and increased the τ value of recovery duration obviously. But inactivation curve of ICa-L was not affected significantly. Conclusion： LBP could inhibit the L- type calcium channel, mainly acting on the activity and recovery processes.

关 键 词：枸杞多糖 全细胞膜片钳 钙电流 

分 类 号：R331.38[医药卫生—人体生理学]