浙江粳稻与日本粳稻品种间遗传差异的SSR分析  

Polymorphism analysis of genetic diversity between Zhejiang japonica varieties and Japan japonica varieties by using SSR markers

作　　者：叶胜海[1] 富田桂 小林麻子 祁永斌[1] 陆艳婷[1] 张小明[1] 

机构地区：[1]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [2]日本福井县农业试验场,日本福井918-8215

出　　处：《浙江农业学报》2008年第6期424-427,共4页Acta Agriculturae Zhejiangensis

基　　金：浙江省重大科技攻关(011102471,2005C22016);浙江省自然科学基金重点项目(Z305650)

摘　　要：从424对分布于水稻12条染色体的SSR引物中筛选出44对用于12份日本粳稻和12份浙江粳稻的多态分析,44对引物多态性筛选结果表明,19对引物在12份日本粳稻间有多态,15对引物在浙江粳稻间有多态,44对引物对两个地区品种的多态检出率分别为43.2%和34.1%。品种间引物多态性分析结果表明,日本粳稻和浙江粳稻的品种(系)内平均多态检出率分别为50.5%和38.7%。UPGMA聚类分析表明,日本粳稻间相似系数在0.5以上,且分为两大类;而浙江粳稻相似系数也在0.5以上且为同一大类。说明浙江粳稻间的遗传差异与日本粳稻间的差异相仿或低于日本粳稻。Forty-four pairs of SSR primers selected from 424 pairs on 12 chromosomes were apphed on polymorphism analysis for japonica rice varieties, which were 12 from Japan and 12 from Zhejiang in china. Results showed that nineteen pairs of primers were polymorphic for the twelve varieties from Japan and fifteen pairs of primers for another twelve varieties from Zhejiang, accounting for 43.2 % and 34.1%, respectively. Polymorphism analysis for varieties showed that average polymorphism ratio among Japan varieties was 50.5% and 38.7% for those among Zhejiang varieties. Cluster analysis revealed that coemcient similarity was above 0.5 and two branches were clustered among Japan varieties, while coefficient similarity was above 0.5 among Zhejiang varieties. The results suggested that genetic diversity among Zhejiang varieties was similar or lower than that among Japan varieties.

关 键 词：粳稻 SSR 多态性 遗传差异 

分 类 号：S511.22[农业科学—作物学]