密骨胶囊和健脾方对去卵巢大鼠骨结构和力学性能的影响  

The effect of MiGu capsule and strengthening spleen prescription to bone structure and bone bio-mechanical property on ovariectomy rats

作　　者：王立童[1] 赵咏芳[1] 王翔[1] 徐宇[1] 陈元川[1] 詹红生[1] 

机构地区：[1]上海中医药大学附属曙光医院上海市中医药研究院骨伤科研究所

出　　处：《中国骨质疏松杂志》2009年第1期27-31,共5页Chinese Journal of Osteoporosis

基　　金：“中医骨伤科学”国家重点学科和上海市重点学科项目(100508,T0303);国家自然科学基金资助项目(30371793,30300459,30472222,30500674);教育部新世纪优秀人才支持计划(NCET040438);教育部博士点基金(20040268012);上海市曙光计划(03SG61);上海市科委重点项目(04JC14071);上海市科委启明星计划课题资助(04qmx1434)

摘　　要：目的观察密骨胶囊、健脾方对大鼠骨结构、生物力学性能的影响，探讨补肾、健脾不同功效的中药复方，对骨结构、生物力学性能作用可能存在的差异性。方法复制切卵巢大鼠骨质疏松模型，随机分为假切组、模型组、密骨胶囊组和健脾方组，术后12周开始灌胃，灌胃12周后取材。测定股骨骨密度、骨生物力学，观察骨组织形态学、骨超微结构。结果密骨胶囊能提高切卵巢大鼠股骨骨密度（P〈0．05），提高骨生物力学性能（P〈0．05），改善骨的微结构；健脾方能维持大鼠股骨骨密度（P〉0．05），提高骨生物力学性能（P〈0．05），明显改善骨的微结构。结论补肾中药复方对骨量的提高、生物力学性能改善方面较健脾方显著，而健脾中药复方较之在骨微结构的改善方面更有优势。Objective The study is to observe the effect of MiGu Capsule and Strengthening Spleen prescriptions on bone structure and bone biomechanics by using therapeutic drug delivery intervention osteoporosis model of Ovariectomized Rat and Chinese patent medicine complex prescriptions with different therapeutic principles such as MiGu Capsule and Strengthening Spleen prescriptions in the guidance of different principles such as invigorating the kidney and Strengthening the spleen. Methods All rats took Ovariectomy operation and were randomly divided into four groups, pseudo-resection group, model group, MiGu Capsule group and Strengthening Spleen prescriptions group. Drug delivery 12 weeks later. Stomach lavage lasts for 12 weeks. Then, to observe the effect of medicine on the rats＇ bone mineral density, bone biomechanics, bone tissue morphology, bone uhramicrostructure. Results MiGu Capsule can enhance bone mineral density （ P 〈 0.05 ）, increase bone biomechanical property （P 〈 0.05）, improve bone structure and bone ultramicrostructure, Strengthening Spleen prescriptions can enhance bone mineral density （P 〉 0.05 ）, increase bone bio-mechanical property （P 〈 0.05 ）, better effect on improving bone structure and bone ul Conclusion MiGu Capsule have a better effect on enhancing bone mineral density and bone bio-mechanical property, Strengthening Spleen prescriptions has a better effect on improving bone structure and bone ultramicrostructure.

关 键 词：补肾 健脾 切卵巢大鼠 骨结构 骨生物力学 

分 类 号：R274[医药卫生—中医骨伤科学]