油茶花药培养褐化愈伤组织的组织化学分析  

Biochemical Analysis of Browning Callus in Oil-Tea Anther Culture

作　　者：闻丽[1] 张日清[1] 刘友全[1] 李建安[1] 

机构地区：[1]中南林业科技大学,湖南长沙410004

出　　处：《经济林研究》2008年第4期34-37,共4页Non-wood Forest Research

基　　金：国家自然科学基金项目(30471418);湖南省教育厅重点项目"湘西坡耕地经济林生态种植模式的研究"(03A056)

摘　　要：为了找到油茶花药培养中愈伤组织褐化与生理生化指标之间的关系,了解褐化的本质原因,从而确定更加有效的培养技术措施,以普通油茶花药培养中褐化的愈伤组织为材料进行了组织化学分析。结果表明:无褐化愈伤组织的细胞含淀粉、脂滴相对较多,而褐化愈伤组织细胞的含量相对较少;褐化愈伤组织的细胞含单宁类物质、过氧化物酶含量相对较多,而无褐化愈伤组织的细胞含量相对较少。In order to find the relationship between callus browning and physiological and biochemical indexes, to learn essential reason of browning, and find some efficient culture measures, browning callus anther culture was analyzed by biochemical methods, taking Camellia oleifera Abel. as material. The results showed that contents of starch and lipid in normal callus cells were higher than that in browning ones ; contents of tannin and peroxidase in cells of browning callus were higher than that in normal ones.

关 键 词：经济林学 油茶 花药培养 愈伤组织 褐化 组织化学分析 

分 类 号：S794.4[农业科学—林木遗传育种]