CML细胞抗原相关TCR Vα13/Vβ21和Vα18/Vβ21寡克隆T细胞及其CDR3序列特点  

CML cell antigens associated TCR Vα13/ Vβ21 and Vα18/Vβ21 oligoclonal T cell and the feature of its CDR3 sequence

作　　者：查显丰[1] 李扬秋[1,2] 陈少华[1] 杨力建[1] 卢育洪[1] 张涛[1] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2008年第6期538-542,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家高技术发展计划(863)资助项目(2006AA02Z114);国家自然科学基金专项基金项目(30424003);广东省自然科学基金项目(05103293);血液病学国务院侨办重点学科建设基金项目

摘　　要：目的:分析慢性粒细胞白血病(CML)病人的克隆性增殖T细胞及其CDR3序列的特点。方法:利用RT-PCR和基因扫描分析1例CML患者外周血单个核细胞中的TCR Vα和Vβ基因的互补决定区(CDR3),了解各Vα和Vβ亚家族的限制性表达情况和T细胞克隆性增殖特点,寡克隆的PCR产物再进行序列分析。结果:该病人外周血T细胞表达18个TCR Vα和12个TCR Vβ亚家族,其中Vα13、Vα18、Vβ1和Vβ21亚家族呈寡克隆性。CDR3序列分析获得3个TCR克隆基因序列,分别为Vα13NJα49、Vα18NJα50和Vβ21NDβNJβ2.7。结论:获得1例CML病人外周血克隆性增殖αβ+T细胞的3个TCR基因CDR3序列,提示病人可能存在与CML细胞抗原相关的Vα13/Vβ21或Vα18/Vβ21T细胞克隆。Aim： To investigate the clonal expansion and the feature of CDR3 sequence of T ceils in the patient with chronic myelogenous leukemia （CML）. Methods： The complementary determining region 3 （CDR3） of TCR Vα and Vβ subfamilies genes were analyzed using RT-PCR and genescan techniques in peripheral blood mononuclear ceils （PBMCs） from the a case with CML, to detect the usage and clonality feature of TCR Vα and Vβ subfamilies. The oligoclonal PCR products were analyzed by sequencing to define the sequence of CDR3. Results： 18 Vα and 12 Vβ subfamily .T cells could be identified in the CML case, and the prodcuts from Vα13, Vα18, Vβ1 and Vβ21 subfamilies displayed oligoclonal expansion. The CDR3 sequences from 3 oligoclonal TCR genes were identified in Vα13NJα49, Vα18NJα5 and Vβ21NDβNJβ2.7 respectively. Conclusion： 3 TCR CDR3 sequences from clonal expansion αβ ＋ T cell in PBMCs from the CML case were identified, suggesting that it might exist TCR Vα13/Vβ21 or Vα18/Vβ21 oligoclonal T cell specific for CML cell antigens.

关 键 词：慢性粒细胞白血病 T细胞受体 基因扫描 CDR3 克隆性 

分 类 号：R392.11[医药卫生—免疫学]