高效液相色谱法测定利节散中三七皂苷R1和人参皂苷Rg1的含量  

Determination of notoginsenoside R1 and Ginsenoside Rg1 content in Lijiesan by HPLC

作　　者：孙巍 任文新[2] 

机构地区：[1]吉林市第五医院,吉林吉林132001 [2]吉林市食品药品检验所,吉林吉林132001

出　　处：《中国中医药杂志》2008年第12期1-4,共4页

摘　　要：目的：建立利节散的含量测定方法。方法：采用高效液相色谱法。色谱柱：Agilent Extend—C18（4．6mm×250mm，5μm），流动相：乙腈-0．3％磷酸溶液（19：81，V／V），流速：1．0ml·min^-1，检测波长：203nm，柱温：40℃。结果：三七皂苷R1在0．03～0．90μg范围内线性关系良好（r=0．9999）；平均加样回收率为100．00％，RSD=1．3％（n=5）；人参皂苷Rg1在0．10～3．00μg范围内线性关系良好（r=1．0000）；平均加样回收率为99．50％，RSD=0．6％（n=5）。结论：该方法简便易行，结果准确可靠，可适用于利节散的质量控制。Objective： To set up a method for quality control of Lijiesan. Method：HPLC method was developed to quantitative determination. COLUMN C18-Agilent Extend（4.6mm× 250mm, 5 μ m）.The mobilic phase was acetonifrile-0.3%phosphoric acid solution （19：81V/V） as mobilic phase. The column temperature was 40℃, the wavelength for detection was 203nm, flow rate was 1.0ml · min^-1. Results：The notoginsenoside R,average of recovery rate was 100.00%, and RSD was 1.3%（n=5）; The ginsenoside Rg, average of recovery rate was 99.50%, and RSD was 0.6%（n=5）. C The method is simple, accurate and suitable for its assaying.

关 键 词：高效液相色谱法 利节散 三七皂苷R1 人参皂苷RG1 含量测定 

分 类 号：R927.2[医药卫生—药学]