快速检疫中培养松材线虫的冷藏真菌菌种筛选  

Selection of the cold-stored fungus species for culturing Bursaphelenchus xylophilus in rapid quarantine

作　　者：李海燕[1] 黄北英[1] 熊惠龙[1] 宋玉双[1] 

机构地区：[1]国家林业局森林病虫害防治总站,沈阳110034

出　　处：《林业科学》2008年第12期157-162,共6页Scientia Silvae Sinicae

基　　金："十五"攻关项目子专题:松材线虫病快速检疫技术研究资助

摘　　要：A long procedure is needed to quarantine pinewood nematodes because only the dispersal third-stage larvae could be isolated and it cannot be identified directly before being cultured to adult nematodes. To shorten the procedure, the effects of different temperatures and culture durations on the development of B. xylophilus larvae were studies with three fungus species as the culture media. The result showed that temperature had differential influence on development of the larvae in the three fungus media, and the influence was not significant. There was significantly different effect of the cold-stored fungi media on the larvae development. Fusarium sp. was the best, Pestalotia sp. was the second, Botrytis cinerea was the worst in promoting larvae development. Cold-stored B. cinerea was not suitable for the rapid quarantine of the nematode. When the L_Ⅲ larvae of B.xylophilus were abundant the quarantine could be accomplished in 48 h by culturing the L_Ⅲ larvae with cold-stored Fusarium sp. or Pestalotia sp. at 30A long procedure is needed to quarantine pinewood nematodes because only the dispersal third-stage larvae could be isolated and it cannot be identified directly before being cultured to adult nematodes. To shorten the procedure, the effects of different temperatures and culture durations on the development of B. xylophilus larvae were studies with three fungus species as the culture media. The result showed that temperature had differential influence on development of the larvae in the three fungus media, and the influence was not significant. There was significantly different effect of the cold-stored fungi media on the larvae development. Fusarium sp. was the best, Pestalotia sp. was the second, Botrytis cinerea was the worst in promoting larvae development. Cold-stored B. cinerea was not suitable for the rapid quarantine of the nematode. When the LⅢ larvae of B. xylophilus were abundant the quarantine could be accomplished in 48 h by culturing the LⅢ larvae with cold-stored Fusarium sp. or Pestalotia sp. at 30 ℃

关 键 词：松材线虫 分散型LⅢ幼虫 冷藏真菌培养 快速检疫检验 

分 类 号：S763[农业科学—森林保护学]