转Bt基因健杨R-94对膜肩网蝽和2种潜叶蛾的抗性  

Resistance of R-94 Bt-transgenic Poplar to Hegesidemus habrus(Heteroptera:Tingidae) and Two Leaf-miner Species

作　　者：魏建荣[1] 李淑梅[2] 陈丛梅 魏红 苏衍修 杨忠岐[1] 李广武[1] 韩一凡[2] 

机构地区：[1]国家林业局森林保护重点实验室中国林业科学研究院森林生态环境与保护研究所,北京100091 [2]中国林业科学研究院林业研究所,北京100091 [3]河南省濮阳市林业科学研究所,濮阳457000

出　　处：《林业科学》2008年第12期163-167,共5页Scientia Silvae Sinicae

基　　金：中国科学院“八五”科技攻关项目资助

摘　　要：There are many reports about resistance of transgenic Bt poplars to lepidopteran defoliators. Whether the poplars can resist haustellate mouth part pests and leaf-miners remains unclear. This study chose transgenic Bt poplar, Populus euramericana cv. Robusta’(R-94), as the material to investigate its resistance to a haustellate mouth part pest, Hegesidemus habrus, and two leaf-miner species, Leucoptera susinella (Lepidoptera: Lyonetiidae) and Lithocolletis paslorella (Lepidoptera: Gracillariidae) in both field and laboratory. Results showed that R-94 poplar had strong resistance to H. habrus in fields. Injury rates of R-94 leaves were respectively only 1.08% in pure stands and 1.1% in mixed stands respectively, while injury rate of 107 poplar (a non-transgenic variety) was 16.3% in the mixed stands. When H. habrus was fed with R-94 leaves in laboratory, the livability of nymphae was 19.9% after 7 days of feeding. However, the livability of nymphae reached to 56.8% after 7 days of feeding with leaves of 107 poplars. When H. habrus was fed with R-94 and 107 poplar leaves together, the insects significantly preferred 107 poplar leaves to R-94 poplar ones. Besides H. habrus, R-94 poplar also showed strong resistance to two leaf-miners species. Therefore, R-94 poplar not only has strong resistance to lepidopteran pest, but also has similar resistance to haustellate mouth part pests and leaf-miners.There are many reports about resistance of transgenic Bt poplars to lepidopteran defoliators. Whether the poplars can resist haustellate mouth part pests and leaf-miners remains unclear. This study chose transgenic Bt poplar, Populus euramericana cv. Robusta＇ （R-94）, as the material to investigate its resistance to a haustellate mouth part pest, Hegesidemus habrus, and two leaf-miner species, Leucoptera susinella （ Lepidoptera ： Lyonetiidae） and Lithocolletis paslorella （Lepidoptera： Gracillariidae） in both field and laboratory. Results showed that R-94 poplar had strong resistance to H. habrus in fields. Injury rates of R-94 leaves were respectively only 1.08% in pure stands and 1.1% in mixed stands respectively, while injury rate of 107 poplar （a non-transgenic variety） was 16.3 % in the mixed stands. When H. habrus was fed with R-94 leaves in laboratory, the livability of nymphae was 19.9% after 7 days of feeding. However, the livability of nymphae reached to 56.8% after 7 days of feeding with leaves of 107 poplars. When H. habrus was fed with R-94 and 107 poplar leaves together, the insects significantly preferred 107 poplar leaves to R-94 poplar ones. Besides H. habrus, R-94 poplar also showed strong resistance to two leafminers species. Therefore, R-94 poplar not only has strong resistance to lepidopteran pest, but also has similar resistance to haustellate mouth part pests and leaf-miners.

关 键 词：转Bt基因健杨 抗虫性 膜肩网蝽 杨白潜叶蛾 柳细蛾 

分 类 号：S763.3[农业科学—森林保护学]