流产患者外周血激活素结合蛋白水平变化的研究  

Analysis of follistatin levels in sera from patients with abortion

作　　者：霍德胜[1] 汪丽[2] 陈秀华[3] 王轶楠[1] 方琳[1] 刘海岩[2] 崔雪玲[1] 闫枫[1] 柳忠辉[1] 

机构地区：[1]吉林大学白求恩医学院免疫学教研室,吉林长春130021 [2]吉林大学中日联谊医院,吉林长春130033 [3]吉林化工医院妇产科

出　　处：《中国实验诊断学》2009年第1期91-93,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省杰出青年基金项目(4-20-5)

摘　　要：目的探讨正常妊娠女性及流产患者外周血及绒毛组织激活素结合蛋白(FS)水平变化。方法采用酶联免疫吸附试验(ELISA)检测外周血FS水平,实时定量PCR检测绒毛组织FS mRNA表达水平。结果正常妊娠女性外周血FS水平变化随孕期增加而增加;流产患者外周血FS水平明显低于正常早期妊娠女性外周血FS水平,统计学比较差异显著,P<0.01;流产患者绒毛组织FS mRNA表达水平也明显低于正常早期妊娠女性绒毛组织FS mRNA表达水平,统计学比较差异显著,P<0.01。结论流产患者外周血FS水平明显降低,检测外周血FS水平变化可能有助于流产诊断。Objective To investigate change of follistatin levels in sera and chofionie villi from patients with abortion and normal pregnant women. Methods The levels of follistatin in sere were detected by ELISA. The expressions of follistatin mRNA in chorionic villi were examined with real-time quantitative PCR. Results The levels of follistatin in sera of normal pregnant women increased gradually with the duration of pregnancy, whereas the levels of FS in sera of abortion patients were lower than that of normal pregnant women, which showed significant difference （ P 〈 0.01） .Furthermore,the expressions of follistatin mRNA in chorionic villi from abortion patients also showed lower than that of normal pregnant women, which had significant difference （ P 〈 0.01）. Conclusion Compared with normal pregnant women,the levels of follistatin in sera of abortion patients decreased significantly,so the levels of follistatin in serum maybe provide useful tools for abortion diagnosis.

关 键 词：激活素结合蛋白 酶联免疫吸附试验 流产 

分 类 号：R714.21[医药卫生—妇产科学]