碳源类型对低温条件下生物反硝化的影响  

Effects of Carbon Source Types on Denitrification Performance at Low Temperature

作　　者：殷芳芳[1] 王淑莹[1] 昂雪野[2] 侯红勋[1] 彭永臻[1] 王伟[1] 

机构地区：[1]北京工业大学水质科学与水环境恢复工程重点实验室,北京100124 [2]大连民族学院,大连116600

出　　处：《环境科学》2009年第1期108-113,共6页Environmental Science

基　　金：“十一五”国家科技支撑重点项目(2006BAC19B03);北京市教委科研基地-科技创新平台项目;北京市属高等学校学术创新团队项目;北京工业大学研究生科技基金项目(ykj-2007-1518)

摘　　要：为了研究低温环境下反硝化细菌对各种碳源的直接反应,利用Carrousel氧化沟系统的活性污泥,以甲醇、乙醇、乙酸钠、丙酸钠、葡萄糖、生活污水及内源物质为碳源,在15.4℃±0.8℃低温状态下开展序批式缺氧反硝化试验.结果表明,乙酸钠为碳源时的最大比反硝化速率(maximum specific denitrification rate,MSDR)最高,达6.51 mg/(g.h),但是其反硝化效率(denitrificationyield,DY)最低,只有0.48,而且存在亚硝酸盐积累现象.甲醇为碳源时的MSDR相比其他几种单一碳源要低,只有0.91mg/(g.h),反硝化细菌对甲醇碳源需要一定时间的适应.当不投加任何外碳源时,反硝化细菌能利用自身体内的原生物质进行内源反硝化,其反硝化速率最低.而以经过厌氧发酵的生活污水作为碳源,其MSDR为3.63 mg/(g.h),达到挥发性脂肪酸(volatile fatty acids,VFAs)作碳源时的水平.低温(15.4℃±0.8℃)下的MSDR相对20℃以上的要低许多,而通过不同的碳源补偿均能在一定程度上改善脱氮效果.To investigate the responding behavior of denitrifying bacteria to different carbon source （ i.e. methanol, ethanol, sodium acetate, sodium propionate, glucose, domestic wastewater and endogenous carbon source）, several batch experiments using the sludge from the Carrousel oxidation ditch were carried out at 15.4℃ ± 0.8 ℃ . The results from sodium acetate feeding experiment show the highest maximum specific denitrification rate （MSDR）, which is 6.51 mg/（g·h） . However, the denitrificaion yield, which is 0.48, is at lowest level and an obvious accumulating of nitrite is observed. Compared to the other sole carbon source, a lower MSDR, 0.91 mg/（g·h） is shown when methanol is used as carbon source, which also suggests that the sufficient adaptation time should be promised for denitrifying bacteria. When the reactor is operated without exogenous carbon source, denitrifying bacteria can use the endogenous carbon source as electron donor for denitrification, but the specific denitrification rate is at lowest level accordingly. The MSDR value of 3.63 mg/（g· h） is obtained with fermented domestic wastewater feeding, which is equivalent to the MSDR attained from the test using VFAs as carbon source. Though the MSDR decreases a lot at low temperature, external carbon source addition improves the denitrification performance to some extent definitely.

关 键 词：生物反硝化 碳源 低温 比反硝化速率 

分 类 号：X701.3[环境科学与工程—环境工程]