杂交水稻种子固有细菌群落多样性探究  

Indigenous Bacterial Community Diversity in Hybrid Rice (Oryza sativa L.)Seed

作　　者：刘琳[1] 刘洋[1] 宋未[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《生物技术通报》2009年第1期95-99,111,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30770069);教育部博士点专项科研基金项目(20060028001)

摘　　要：采用传统的分离培养方法和分子生物学技术对我国高产杂交水稻(OryzasativaL.)金优611种子固有细菌进行研究,从而了解其中可培养细菌群落的多样性。对分离得到的91株细菌进行16SrDNA扩增、ARDRA分型和16SrDNA系统发育分析,结果表明,分离得到的91株细菌分属于10个属16个种。其中γ-变形杆菌(Gammaproteobacteria)(53.85%)占据优势地位,其次为α-变形杆菌(Alphaproteobacteria)(20.88%),其它分属放线菌门Actinobacteria(15.39%)及厚壁菌门Firmicutes(9.88%)。其中的泛菌属(Pantoea sp.)和鞘氨醇单胞菌属(Sphingomonas sp.)、假单胞菌属(Pseudomonas sp.)、微杆菌属(Microbacterium sp.)为分离到的优势种群,且在种子这一特殊的生存空间中有4株潜在的新种存在。首次报道了杂交水稻金优611种子具有丰富的微生物群落多样性,为进一步探索植物种子际微生态环境中微生物群落的形成和生态功能提供了基础信息。The indigenous bacteria from hybrid rice（Oryza sativa L.）Jinyou611（F1）seed were isolated and identified by using traditional cultural methods combined with molecular biotechnology to explore bacterial community diversity. ARDRA patterns of amphfied 16S rDNA and phylogenetic analysis based on the partial 16S rDNA sequences,indicated that the 91 strains from Jinyou 611 were clustered into 16 species of ten genera. The most dominant group was Gammaproteobacteria （53.85%）,followed by Alphaproteobacteria （20.88%）. The rest were clustered into Actinobacteria （15.39%）and Firmicutes （9.88%）respectively. The dominant bacteria were Pantoea sp., Sphingomonas sp. Pseudomonas sp. Mierobacterium sp. Additionally,four potential novel species were found. This is the first report about abundant diversity of bacterial community occurred within hybrid rice seeds.

关 键 词：杂交水稻种子 固有细菌 群落多样性 

分 类 号：Q93[生物学—微生物学]