急性特发性血小板减少性紫癜患儿外周血淋巴细胞过氧化物酶体增生物活化受体γmRNA表达及其与血清白细胞介素-2的相关性  

Lymphoblastic PPAR-γ mRNA expression and the relevance with plasma IL-2 in children with acute idiopathic thrombocytopenic purpura

作　　者：金呈强[1] 刘仿[1] 肖红[2] 宋丽[1] 王文涓[1] 

机构地区：[1]广东医学院微生物学与免疫学教研室,广东湛江524023 [2]广东医学院第一附属医院儿科,广东湛江524000

出　　处：《新乡医学院学报》2009年第1期13-15,共3页Journal of Xinxiang Medical University

基　　金：广东省自然科学基金(编号:06028959)

摘　　要：目的检测急性特发性血小板减少性紫癜(ITP)患儿外周血淋巴细胞过氧化物酶体增生物活化受体γ(PPAR-γ)mRNA表达变化及其与血清白细胞介素-2(IL-2)的相关性。方法急性ITP组为急性ITP患儿53例,对照组为同期体检儿童50例;反转录聚合酶链反应法(RT-PCR)检测外周血淋巴细胞PPAR-γmRNA的表达,酶联免疫吸附测定法(ELISA)检测血清中IL-2水平。结果急性ITP组患儿外周血淋巴细胞PPAR-γmRNA表达显著高于对照组(P<0.05),血清中IL-2的含量显著低于对照组(P<0.05);急性ITP患儿血清中的IL-2含量与外周血淋巴细胞PPAR-γmRNA表达呈负相关(r=0.81,P<0.05)。结论急性ITP患儿外周血淋巴细胞PPAR-γmRNA的表达可能抑制了IL-2的生成,PPAR-γ与IL-2可能参与了急性ITP的发病过程。Objective To detect the expression of PPAR-γ mRNA in peripheral blood lymphoblastic and analyse the relevance with plasma IL-2 in children with acute idiopathic thrombocytopenic purpura （ITP）. Methods The expression of PPAR-γ mRNA in peripheral blood lymphoblastic was detected by RT-PCR and plasma IL-2 was detected by ELISA in ITP group （n = 53 ）and control group（ n = 50）. Results The expression of PPAR-γ mRNA in peripheral blood lymphocytes of acute ITP children were significantly higher than the control group（ P 〈0.05 ） ;Plasma IL-2 of acute ITP children were significantly lower than the control group（ P 〈 0.05 ）. There was a negative correlation between plasma IL-2 contents and lymphoblastic PPAR-γ mRNA expression in acute ITP children（ r = 0.81, P 〈 0.05 ）. Conclusion The expression of PPAR-γ mRNA in peripheral blood lymphoblastic may inhibit the production of IL-2;PPAR-γ and IL-2 may be play a part in the pathogenetic process of acute ITP.

关 键 词：急性特发性血小板减少性紫癜 过氧化物酶体增生物活化受体γ 白细胞介素-2 

分 类 号：R554.6[医药卫生—血液循环系统疾病]