毛细管电泳电化学发光用于丙吡胺及其与血浆蛋白结合率的测定  

Determination of Disopyramide and Its Binding Rate with Plasma Protein Studied by Capillary Electrophoresis with Electrochemiluminescence Detection

作　　者：周玲玲[1] 袁吉培[1] 汪尔康[1] 

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022

出　　处：《分析化学》2009年第1期53-56,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20675078);中国科学院(No.KJCX2;YWH11)资助课题

摘　　要：本实验利用透析袋平衡透析、毛细管电泳Ru(bpy)32+电化学发光检测技术测定了丙吡胺和人血浆蛋白的结合率。在恒电位1.3V;进样电压10kV持续10s,分离电压15kV,运行缓冲液30mmol/L磷酸盐缓冲液(pH7.5),检测池中为5mmol/LRu(bpy)32+稀释于50mmol/L磷酸盐缓冲液(pH7.5)中等最优化的条件下,丙吡胺的检出限为10μmol/L(S/N=3)。对蛋白结合率的测定结果表明,人血浆中的药物浓度为1.6～8.2mmol/L,丙吡胺与血浆蛋白的结合是呈线性的,其线性回归方程为y=-0.07+0.93x,线性相关系数r为0.9999,丙吡胺与人血浆蛋白的结合率约为90.4%。The binding rate of disopyramide with human plasma protein was determined using capillary electrophoresis（CE） coupled with tris（2,2＇-bipyridyl） ruthenium（ Ⅱ ） [ Ru（bpy）3^2＋ ] electrochemiluminescence （ECL） detection after equilibrium dialysis. Under optimal conditions（ ECL detection at 1.3 V, electrokinetically injection at 10 kV for 10 s, 15 kV as the separation voltage, 30 mmol/L phosphate buffer at pH 7.5 as running buffer and 5 mmoL/L Ru（bpy）3^2＋ in 50 mmol/L phosphate buffer at pH 7.5 in the detection cell） , the detection limit of disopyramide was 10 μmoL/L（S/N = 3 ）. The results of protein binding indicated that the binding of disopyramide with plasma protein was linear with a correlation coefficient of 0. 9999 when the ranges of concentration of disopyramide in plasma were 1.6 -8.2 retool/L, and the human plasma protein binding rate of disopyramide was 90.4%.

关 键 词：毛细管电泳 电化学发光 丙吡胺 血浆蛋白结合率 平衡透析 

分 类 号：R96[医药卫生—药理学]