麻黄碱的分子烙印聚合物的制备方法及其结合性能  

Preparation and Binding Characteristic of Ephedrine-imprinted Polymers

作　　者：许志锋[1] 邝代治[1] 张复兴[1] 邓芹英[2] 

机构地区：[1]衡阳师范学院化学与材料科学系,衡阳421008 [2]中山大学化学与化学工程学院,广州

出　　处：《应用化学》2009年第1期21-26,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20202015);衡阳师范学院科研启动基金(2006B04)资助项目

摘　　要：用甲基丙烯酸(MAA)或2-乙烯吡啶(2-Vpy)作为功能单体,CHCl3、CH3CN或CH3OH作致孔剂,制备了一系列麻黄碱的分子烙印聚合物(MIP)。平衡吸附实验结果表明,用MAA作功能单体,CHCl3作致孔剂制备的P1对麻黄碱具有明显的选择性,其特异性吸附量ΔCP=91.98μmol/g,烙印因子IF=4.42;用CH3OH作致孔剂时,MIP对模板分子的高吸附量更多来源于非特异性吸附。改变模板分子与功能单体的用量比对MIP的分子识别性能有重要的影响。紫外光谱和1HNMR结果表明,模板分子与功能单体之间依靠静电作用和氢键作用在聚合前的混合溶液中生成1∶2的复合物,MAA与麻黄碱生成的复合物在甲醇、氯仿和乙腈中的平衡离解常数分别为48 450.8、45 813.3和23 418.2(mol/L)-2。A series of Ephedrine-imprinted polymers were prepared with methacrylic acid （MAA） or 2-vinylpyridine（2-Vpy） as functional monomer and chloroform, aeetonitrile or methanol as porogen. The equilibrium binding experiments results show that the imprinted polymer P1, prepared with MAA as the functional monomer, chloroform as the porogen, obviously exhibited a higher selectivity for Ephedrine. The specific binding capacity（ACe） and imprinting factor（IF） of P1 were 91.98 μmol/g and 4. 42, respectively. The polymer prepared with methanol as the porogen showed the highest binding capacity for ephedrine. However, its higher binding capacity mainly originated from the unspecific binding. The recognition ability of the molecularly imprinted polymer was effectively influenced by the molar ratio of the template to functional monomer. The results of UV and I H NMR experiments suggest that a template-monomer complex was formed based on hydrogen bonding and ionic interactions in the pre-polymeric mixture, and the molar ratio of template to monomer in the complex was 1 ： 2. The equilibrium constants of the reactions for producing the templatemonomer complexes in chloroform, acetonitrile and methanol were calculated to be 48 450. 8, 45 813.3 and 23 418.2 （mol/L）^-2, respectively.

关 键 词：分子烙印 麻黄碱 结合能力 紫外光谱 功能单体 

分 类 号：O657.3[理学—分析化学]