间接ELISA技术检测近江牡蛎病原—类立克次体  

Indirect ELISA Technique for Detection of a Rickettsia-like Organism from Oyster,Crassostrea ariakensis

作　　者：孙敬锋[1,2] 毕相东[1] 吴信忠[3] 

机构地区：[1]天津农学院水产科学系天津市水产生态及养殖重点实验室,天津300384 [2]华南师范大学生命科学学院,广东广州510631 [3]浙江大学动物科学学院,浙江杭州310029

出　　处：《水产科学》2009年第1期8-10,共3页Fisheries Science

基　　金：国家自然科学基金资助项目(39970581);广东省自然科学基金资助项目(06300897);中国博士后科学基金资助项目(20060390731)

摘　　要：以近江牡蛎病原—类立克次体为抗原,制备鼠抗血清,利用辣根过氧化酶标记的羊抗鼠血清(HRP-IgG)为酶标二抗,建立了检测近江牡蛎类立克次体的间接ELISA快速检测方法。结果表明,应用间接ELISA技术检测近江牡蛎类立克次体有较高的灵敏度,最低的检测量为50 ng的全菌抗原蛋白量。同时交叉反应试验表明该方法具有较高的特异性。利用建立的间接ELISA技术,对50份自然患病的近江牡蛎鳃组织进行类立克次体检测,阳性率为89%。The indirect enzyme-linked immunosorbent assay（ELISA）was developed for the rapid detection of RLOs by polyclonal antiserum against a rickettsia-like organism from oyster Crassostrea ariakensis produced in the male BALB/e mouse and the horseradish peroxidase-conjugated goat anti-mouse IgG（HRP- IgG）. The ELISA method was favored by high sensitivity, and lowest detection dose of 50 ng RLO protein. The crossreaetion assay showed the ELISA method had the high specificity. In the detection of the diseased oyster gills, 89 % of 50 samples were found to be positive by the indirect ELISA method.

关 键 词：ELISA技术 近江牡蛎 类立克次体 检测 

分 类 号：S917.1[农业科学—水产科学]