两种小车蝗染色体C带核型研究(蝗总科:斑翅蝗科)  

C-banding Karyotypes of Two Species of Oedaleus Fieber in Guangxi(Acridoidea:Oedipodidae)

作　　者：韦仕珍[1] 邓维安[1] 

机构地区：[1]河池学院化生系,广西宜州546300

出　　处：《四川动物》2009年第1期7-10,共4页Sichuan Journal of Zoology

基　　金：广西高校优秀人才资助计划(RC2007034)

摘　　要：采用秋水仙素体内注射法取昆虫精巢,低渗处理,空气干燥制片法制作染色体标本,吉姆萨染色。首次对分布在广西的小车蝗属Oedaleus两个种隆叉小车蝗Oedaleus abruptus(Thunberg)和红胫小车蝗Oedaleus manjiusChang染色体核型和C带带型进行了分析研究。结果表明:两种小车蝗的染色体基数、性别决定机制、染色体组式、染色体着丝粒类型和着丝粒C带等方面有着相同的特征,结构异染色质在染色体组中的总含量也比较接近;染色体是端部着丝粒和染色体都含有着丝粒C带带纹上具有一致性。但在个体大小、相对长度值、性染色体、除着丝粒C带以外的其它C带分布类型方面都有明显差异。所得结果符合传统的形态学分类。研究结果为直翅目的物种亲缘关系、遗传多样性分析提供有价值的生化遗传指标。The metaphase chromosome of Oedipodidae was prepared from kidney by colchicine injection and method of BSG（Bafiumhydroxide/Saline/Giemsa） adopted for C-banding staining and air dried. The karyotype was analyzed. The C- banding karyotypes of Oedaleus manjius Chang and Oedaleus abruptus （Thunberg） in Oedaleus in Guangxi were studied. According to the result of the test, there was obvious difference on individual size, sex chromosome, intercalary and telomere band, but there was the same character on chromosome number, sex determination, genome formula, telocentfic chromosome and centromeric band. The amount of heterchromatin is similar. The results are consistent with the taxon based on traditional morphology, and provided valuable biochemical and genetic indices for the relative relationship and genetic diversity of Orthoptera.

关 键 词：小车蝗属 染色体 核型 C带 广西 

分 类 号：Q96[生物学—昆虫学]