紫草的组织培养及快速繁殖研究  

Tissue Culture and Clone Construction of Lithospermum erythrorhizon Sieb.et Zucc

作　　者：张倩怡[1] 张丽莎[1] 郑宏丽[1] 李永德[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《河南科学》2009年第1期55-58,共4页Henan Science

摘　　要：以紫草的嫩茎为材料,进行了愈伤组织的诱导、愈伤组织的分化、不定芽的生根和生根继代、试管苗的移栽和移植的研究,建立起紫草嫩茎的无性系,达到了快速繁殖的目的.结果表明:MS+BA0.5mg/L+NAA1.8mg/L是诱导嫩茎形成具有分化能力愈伤组织的理想培养基;MS+AgNO31.5mg/L+BA0.3mg/L+NAA0.1mg/L是愈伤组织和不定芽分化的理想培养基;1/3MS+IAA0.4mg/L是试管苗生根培养的理想培养基;移植后试管苗保持了野生植株的各种生物性状,后期出现了生长旺盛、根系发达的特点.The conditions needed in callus induction,differentiation of axillary bud,rooting and transplantation of young stem from Lithospermum erythrorhizon Sieb.et Zucc have been studied,its regenerative clone and rapid propagation line were established. The results showed that the best medium for callus induction was MS+BA0.5 mg/L+ NAA1.8 mg/L with ability of differentiation. The best media of differentiation was MS+AgNO31.5 mg/L+BA0.3 mg/L+ NAA0.1 mg/L,1/3MS+IAA0.4 mg/L was suitable for rooting,regeneration. Field planting showed that the tube spout grew well with roots flourishing.

关 键 词：紫草 组织培养 无性系 

分 类 号：Q943.1[生物学—植物学]