几种有降压作用的植物中芦丁和槲皮素含量测定  

Determination of Rutin and Quercetin in Anti-hypertension Herbal Plants by RP-HPLC Simultaneously

作　　者：孙娟娟[1] 葛红[1] 侯婷婷[1] 于波[1] 

机构地区：[1]辽宁师范大学生物技术与分子药物研发重点试验室,辽宁大连116029

出　　处：《中国现代中药》2009年第1期12-15,共4页Modern Chinese Medicine

基　　金：辽宁省"高等学校优秀人才支持计划"项目(2006R33)

摘　　要：目的:比较具有降压作用的5个科属代表性植物中芦丁和槲皮素含量差异。方法:建立一种反相高效液相色谱法同时测定中药材(连翘、杜仲、槐花、金银花、雪莲花)中芦丁和槲皮素的含量。相同的液相条件下,分别得到芦丁和槲皮素的线性回归方程。结果:2种黄酮化合物线性范围为10～5000ng,平均加样回收率均在97%～102%。2种化合物液相分析精密度和重复性RSD均小于5%,定量限(S/N=10)低于5.0 ng。测得芦丁与槲皮素在不同科植物中的含量并加以比较。结论:该分析方法简单、灵敏、重现性好、结果准确,可对多种药材中2种黄酮类化合物进行分析,可用于多种药材质量标准的制订。Objective： To compare the contents of Rutin and Quercetin in five anti-hypertention herbal plants. Methods： To establish a RP-HPLC method for quantification of Rutin and Quercetin contents in five Chinese herbs （ Forsythia suspensa （Thunb.） vahl. , Sophora japonica L , Saussurea involucrate, Lonicera japonica, Thunb, Eucommia ulmoides oliv） . Regression analyses with the peak area （A） and the concentration of reference substance （C） were carried out under the same HPLC condition. Linear regression equations of Rutin and Quercetin reference substance can be found respectively. Results： The linear range of these flavonoids were 10 -5 000 ng. The average recovery range was 97% - 102%. The RSDs of the two compounds in sensitivity and reproducibility were both less than 5%, the least quantity （S/N = 10） is 5.0 ng. Conclusion： The method is simple, sensitive, reproducible, accurate and applicable for quantification of flavonoids in various Chinese herbs. It can be used for establishing quality standards of Chinese herbs.

关 键 词：反相高效液相色谱 芦丁 槲皮素 含量测定 

分 类 号：R972.4[医药卫生—药品] R284.1[医药卫生—药学]