PTA1单克隆抗体诱导人血小板活化聚集和胞浆Ca^(2+)水平的升高  

PTA1 monoclonal antibody induces human platelet aggregation and intracytoplasmic Ca2+ elevation

作　　者：孙忱[1,2] 金伯泉[1,2] 刘雪松[1,2] 杨琨[1,2] 张婷婷[1,2] 董邦权[1,2] 李家增[1,2] 武怀珠[1,2] 彭林[1,2] 包承鑫[1,2] 武文杰[1,2] 

机构地区：[1]第四军医大学免疫学教研室 [2]中国医学科学院中国协和医科大学血液学研究所

出　　处：《中华血液学杂志》1998年第3期133-137,共5页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：目的：探讨血小板和Ｔ细胞活化抗原１（ＰＴＡ１）诱导人血小板活化聚集和对血小板胞浆Ｃａ２＋水平影响的机制。方法：血小板聚集与ＡＴＰ释放试验及血小板胞浆Ｃａ２＋水平测定。结果：ＰＴＡ１单克隆抗体（ＭｃＡｂ）体外可诱导人血小板活化聚集，ＥＧＴＡ与ＰＧＩ２可以完全抑制ＰＴＡ１ＭｃＡｂ诱导的血小板活化聚集，ＰＴＡ１ＭｃＡｂＦ（ａｂ′）２对ＣＤ９或ＣＤ４１诱导的血小板活化聚集无明显影响。ＰＴＡ１ＭｃＡｂ促进血小板胞浆Ｃａ２＋水平升高。结论：ＰＴＡ１ＭｃＡｂ的刺激作用与血小板膜表面Ｆｃ受体和ＣＤ４１／ＣＤ６１（ⅡｂⅢａ）复合物有关，促进血小板胞浆Ｃａ２＋水平升高为胞外Ｃａ２＋内流与胞浆内Ｃａ２＋储存释放共同作用所致。Objective:To explore the mechanism of PTA1 monoclonal antibody(McAb) induced human platelet aggregation and its effect on intracytoplasmic Ca2+ level.Methods:Platelet aggregation,ATP releasing assay and Pollock's test were used.Results:PTA1 McAb induced human platelet aggregation in vitro,which could be completely inhibited by EGTA and PGI2.F(ab′)2 of PTA1 McAb had no effect on CD9 or CD41 induced platelet activation and aggregation.PTA1 McAb enhanced platelet intracytoplasmic Ca2+ elevation.Conclusion:PTA1 McAb inducing platelet aggregation is related to platelet Fc receptor and CD41/CD61(Ⅱb/Ⅲa) complex,and the induced platelet intracytoplasmic Ca2+ elevation is resulted from Ca2+ influx and releasing of intracytoplasmic Ca2+ storage.

关 键 词：T细胞活化抗原 PTA1 血小板聚集 钙 单克隆抗本 

分 类 号：R392－33[医药卫生—免疫学] R331.143[医药卫生—基础医学]