鸡堆型艾美耳球虫特异性单抗轻、重链可变区基因的克隆与序列测定  

Cloning and nucleotide sequencing of heavy and light chain variable genes from species-specific monoclonal antibodies against E.acervulina

作　　者：赵月兰[1] 郭红斌[1] 秦建华[2] 康桂英[3] 张磊[1] 包永占[2] 

机构地区：[1]河北农业大学中兽医学院,河北定州073000 [2]河北农业大学动物科技学院,河北保定071000 [3]内蒙古民族大学动物科技学院,内蒙古通辽028000

出　　处：《中国兽医学报》2009年第1期45-48,共4页Chinese Journal of Veterinary Science

基　　金：河北省自然科学基金资助项目(303226)

摘　　要：将已扩增出的鸡堆型艾美耳球虫特异性单抗轻、重链可变区基因进行纯化,并用纯化的2基因片段与pMD-18T载体连接,将重组载体转化于感受态细胞JM109,筛选出阳性重组子,提取阳性重组子质粒并进行测序。得到单抗轻、重链可变区的基因序列。轻链基因为312 bp,重链基因为381 bp,为单链抗体基因的构建及免疫毒素的构建奠定了基础。To study the function of small molecule antibody, heavy and light chain variable genes which were amplified from species-specific monoclonal antibodies against E. acervulina were purified and cloned into clone vector pMD18-T by gene recombination techniques. The recombinant plasmid was transformed into E. coli JM109. By screening,several recombinants that had been inserted with the target fragments were obtained. The positive clones containing recombination were idendified by the gene sequencing. The result demonstrated that light chain variable genes was consisted of 315 bp. The heavy chain variable genes is 381 bp.

关 键 词：鸡堆型艾美耳球虫 单抗 轻链 重链 可变区基因 克隆 测序 

分 类 号：G852.723[文化科学—民族体育]