鸡组织中地克珠利和妥曲珠利残留HPLC检测方法的建立  

Simultaneous determination of residues of diclazuril and toltrazuril in chicken tissues by high performance liquid chromatography

作　　者：施祖灏[1] 朱良强[2] 卢运站[1] 祁克宗[1] 彭开松[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽省兽医工作站,安徽合肥230022

出　　处：《中国兽医学报》2009年第1期79-81,109,共4页Chinese Journal of Veterinary Science

基　　金：“十一.五国家科技支撑计划”课题分课题(2006BAK02A08);安徽省“十五”科技重大攻关项目(0400303041);安徽省优秀青年基金(04041042)

摘　　要：建立了同时检测鸡组织中地克珠利和妥曲珠利残留的高效液相色谱。经乙腈提取，正己烷脱脂，旋转蒸发浓缩后，以0．05mol／L磷酸／三乙胺（pH3．0）：乙腈（40：60）作为流动相，反相高效液相色谱-紫外检测法检测，检测波长240nm。鸡组织中地克殊利和妥曲珠利的回收率分别为92．0％～102．0％和83．4％～89．0％，变异系数为5．9％～12．2％，地克珠利和妥曲珠利的检测限分别为0．012mg／kg和0．010mg／kg，在0．05～110mg／L质量浓度范围内呈良好的线性相关。该法样品处理简单，可同时检测地克珠利和妥曲珠利的残留，且准确度和精密度均符合残留分析的要求。A simple and rapid determination of anticoccidial drug residues, diclazuril and toltrazuril, in chicken tissues has been developed. Diclazuril and toltrazuril were extracted with acetonitrile from chicken liver,muscle,and kidney. The extract was rinsed with N-hexane saturated with acetonitrile and then evaporated. The residue was dissolved in 1.0mL of mobile phase, then determined by HPLC on reversed-phase C18 column with 0.05 mol/L phosphate acid/acetate buffer-acetonitrile （40 ： 60） as the mobile phase. At the linear correlation 0.01 - 5.0 mg/L,the mean recoveries （n= 5） of diclazuril and toltrazuril spiked in chicken tissues were 92.0%- 102.0% （CV 5.9%- 12.2%） and 83.4%- 89.0% （CV 6.9%- 8.6%） ,respectively. The detection limits of diclazuril and toltrazuril were 0. 005 and 0. 004 mg/kg, respectively. This method meets the demand of residues determination of diclazuril and toltrazuril.

关 键 词：高效液相色谱 鸡组织 地克珠利 妥曲珠利 残留检测 

分 类 号：S852.723[农业科学—基础兽医学]