抗体和寡核苷酸双标记纳米金生物探针的制备及生物学特性  

Preparation and Biological Characterization of Antibody and DNA Dual-labeled Gold Nanoparticles

作　　者：李富荣 乔飞燕[2] 孔小丽 周汉新 齐晖 任莉莉 

机构地区：[1]暨南大学第二临床医学院(深圳人民医院)临床医学研究中心,深圳518020 [2]兰州大学化学化工学院,兰州730000

出　　处：《高等学校化学学报》2009年第1期60-63,共4页Chemical Journal of Chinese Universities

基　　金：国家“八六三”计划项目(批准号:2003BA310A23);深圳市科技局计划项目(批准号:2004B110)资助

摘　　要：纳米金通过静电吸附抗体,与寡核苷酸共价结合制备双标记纳米金生物探针,比较了双标记纳米金生物探针和单标记抗体IgG或ss-DNA的稳定性和反应性.结果表明,在水溶液中纳米金由于ss-DNA的结合使IgG抗体的吸附能力明显改善,IgG的吸附也影响二硫苏糖醇(DDT)对ss-DNA的解离作用.双标记纳米粒上覆盖(50±15)条ss-DNA和(10±2)条IgG,较单标记ss-DNA纳米金上的(70±15)条要少.斑点免疫和杂交实验证明,纳米金表面标记的IgG和ss-DNA具有良好生物学活性.双标记纳米金生物探针在超微量蛋白质的检测中具有应用价值.Gold nanoparticles labeled by both antibody （IgG） and single stranded DNA （ss-DNA） were synthesized and characterized. The stability and reactivity of the dual-labeled nanopartieles were compared with the conventional IgG or ss-DNA modified nanoparticles. It was found that the IgG adsorption significantly improved the stability of the nanoparticles in aqueous solution, which is beneficial for attaching ss-DNA. The presence of IgG also effectively prohibits the desorption of ss-DNA against dithiothreitol（DTT） displacement. The coverage on dual-labeled nanoparticles was found to be （ 50 ±15 ） ss-DNA/nanoparticle and （ 10 ±2 ） IgG/nanoparticle, respectively, compared to the value of （70 ±15） ss-DNA/nanoparticle of only ss-DNA-labeled gold nanoparticles. Dot-immuno and cross-linking experiments confirmed that both the IgG and ss-DNA retained their bioactivity on the nanoparticle surface. The dual-labeled nanoparticles have potential to be used as novel bio-probes for ultrasensitive detection.

关 键 词：金纳米粒子 生物探针 寡核苷酸 抗体 

分 类 号：O629.7[理学—有机化学]