非肥胖糖尿病鼠Sca-1^+细胞的分离和纯化  

Isolation and purification of Sca-1^+ cell from nonobese diabetic (NOD ) mice

作　　者：赵艳[1] 高峰[1] 吕平[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022 [2]华中科技大学同济医学院附属协和医院血管外科

出　　处：《临床血液学杂志》2009年第1期40-42,共3页Journal of Clinical Hematology

基　　金：湖北省卫生厅重点项目(No:JX2A07)

摘　　要：目的:分离和纯化非肥胖糖尿病(NOD)鼠的造血干细胞。方法:运用针对小鼠干细胞表面特异表达的干细胞抗原-1(Sca-1),采用磁性细胞分选方法(MACS)分离NOD鼠骨髓造血干细胞,并应用流式细胞术(FACS)监测分选效果。结果:纯化后Sca-1+细胞纯度平均为83.01%,细胞回收率为52.48%~57.64%,细胞活力在90%以上。结论:MACS可以从NOD鼠骨髓中分离出Sca-1阳性造血干细胞,细胞纯度可达80%以上。Objective：To isolate and purify hematopoietic stern cell from non obese diabetic mice. Method：The bone marrow hematopoietic stem cells were isolated from non-obese diabetic mice by means of magnetic activated cell sorting（MACS） using the stem cell antige-1（Sca-1） expressed on the surface of hematopoietic stem cells. Flow eytometry（FACS） was used to monitor the efficiency of the isolation. Result：The purity of Sea-1^＋ ceils amounted to 83.01% in average after the purification, and the recovery rate was from 52.48% to 57.64%, the cell viability was above 90%. Conclusion：MACS can be used to isolate the Sea-1^＋ hematopoietic stem cells from NOD mice and the purity of the isolated cell proportion was more than 80%.

关 键 词：糖尿病 NOD鼠 骨髓造血干细胞 免疫磁珠法 流式细胞术 

分 类 号：R587[医药卫生—内分泌]