野猪、家猪及野家杂种猪calpain3基因的多态性分析  

The polymorphic analysis on CAPN3 in wild boar,swine and hybrid swine

作　　者：关庆芝[1] 杨秀芹[1] 郭丽娟[1] 刘娣 

机构地区：[1]东北农业大学动物科技学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院,黑龙江哈尔滨150086

出　　处：《黑龙江畜牧兽医》2009年第1期10-11,共2页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省杰出青年基金项目(JC-05-19);黑龙江省科技攻关项目(GB05B106)

摘　　要：Calpain3(CAPN3)在肌肉组织中特异表达,其活性显著影响宰后肉的嫩化,研究以野猪、家猪及野家杂种猪为研究对象,对其上的一个点突变进行了基于限制性内切酶H incⅡ的PCR-RFLP分析。结果表明:2个等位基因在野猪、家猪中的分布存在着极显著的差异,在长白、大白、杜洛克这3个欧洲引进猪种中没有检测到B等位基因;民猪共检测了35头,只发现了3个AB杂合体,其余都是AA型;而野猪却以B为优势基因,B等位基因频率明显高于A等位基因,结合品种(品系)特性说明该突变可能与瘦肉率和嫩度有关。Calpain 3, specific expressed in muscle, have a role in postmortem tenderization. The polymorphism was analyzed by PCR - RFLP method based on restriction endonuclease Hinc Ⅱ among wild boar, swine and their hybrid swine. The result showed that the distribution of two alleles is extremely significant difference among breeds. No allele B is detected in three imported pig breeds such as Landrace, Yorkshire and Duroc. In the Min pig,only three AB genotype is found and the others are AA homozygote. While allele B is dominant in wild boar. The mutation may be relavant to meat percentage and tenderness.

关 键 词：calpain3 野猪 家猪 野家杂种猪 限制性内切酶HincⅡ 

分 类 号：S828[农业科学—畜牧学]